Effect of peptide loading and surfactant concentration on the characteristics of physically crosslinked hydrogel.
Effect of peptide loading and surfactant concentration on the characteristics of physically crosslinked hydrogel.
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肽负载和表面活性剂浓度对物理交联水凝胶特性的影响。
DOI：
10.1081/pdt-100102038
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发表时间：
2000
影响因子：
3.4
通讯作者：
Bostanian,LA
中科院分区：
文献类型：
--
作者：
Mandal,TK;Bostanian,LA
本研究的目的是考察不同载药量和不同浓度的表面活性剂(十二烷基硫酸钠[SLS])对模型多肽(牛血清白蛋白[BSA])释放特性的影响，并研究水凝胶骨架[聚乙烯醇(PVA)]的溶胀性质的净影响。在不使用化学交联剂的情况下，采用冻融法制备了PVA水凝胶。蛋白质载量对药物释放的影响在三个水平(0.65、1.3和2%)进行考察，而SLS对药物释放的影响在四个水平(0、0.07、0.13和0.26%)进行考察。含0.65%BSA的水凝胶达到平衡溶胀的基线时间为48小时，当蛋白质负载量增加到2%时，平衡溶胀时间显著缩短。BSA浓度增加的净影响导致BSA从水凝胶基质中更快地溶解。加入SLS后，PVA溶液的平衡溶胀比从21%降至10%，平衡溶胀程度减小，但达到平衡溶胀的时间延长。该研究通过改变给药系统中两种基本成分，即药物和表面活性剂的浓度，为水凝胶配方的开发提供了机理基础。
The purpose of this study was to investigate the effect of varying drug load and concentration of a surfactant (sodium lauryl sulfate [SLS]) on the release characteristics of a model peptide (bovine serum albumin [BSA]), and study the net effects of the swelling properties of the hydrogel matrix [poly(vinyl alcohol) (PVA)]. The PVA hydrogel was prepared by a freeze-thaw process in the absence of a chemical crosslinking agent. The effect of protein loading on drug release was examined at three levels (0.65, 1.3, and 2%), whereas the effect of SLS was studied at four levels (0, 0.07, 0.13, and 0.26%). The baseline time for reaching equilibrium swelling was 48 hr for the hydrogel containing 0.65% BSA, and the equilibrium swelling time decreased significantly as the protein load was increased to 2%. The net effect of increased BSA concentrations resulted in faster BSA dissolution from the hydrogel matrix. The equilibrium-swelling ratio decreased from 21 to 10% when SLS was added to the PVA solution, which resulted in a reduction in the extent of equilibrium swelling; however, the time to reach equilibrium swelling was increased. The investigation provided a mechanistic basis toward the development of a hydrogel formulation by altering the concentration of two fundamental components, i.e., drug and surfactant, within the delivery system.