Culturing and Mating of Aspergillus fumigatus.
Culturing and Mating of Aspergillus fumigatus.
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DOI：
10.1002/cpmc.87
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发表时间：
2019-09
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中科院分区：
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烟曲霉菌是一种机会致病性人类真菌病原体，能够在免疫系统受损的患者中引起侵袭性曲霉病。这种真菌长期以来被认为是一种纯粹的无性生物。然而，2009年报告了性周期，并描述了在实验室条件下诱导交配的方法。性周期的存在现在为真菌的经典遗传分析提供了一个有价值的工具，例如可以确定感兴趣的性状在本质上是单基因还是多基因。例如，目前正利用性周期来确定具有医学重要性的性状的遗传基础，如对唑类抗真菌剂和毒力的抗性，并描述所涉及的基因。性周期也可以用来评估分离株之间基因流动的可能性。这是一个开放获取的条款下的知识共享署名许可证，允许使用，分发和复制在任何媒体上，只要原始作品是适当的引用。本单元描述了培养A.以及用于诱导该物种的相容性MAT 1 - 1和MAT 1 - 2分离株之间的有性生殖。该单位还提供了收获有性结构，分离单孢子后代和确认是否发生有性重组的工作方法。©作者。这是一个开放获取的条款下的知识共享署名许可证，允许使用，分发和复制在任何媒体上，只要原始作品是适当的引用。
Aspergillus fumigatus is an opportunistic human fungal pathogen, capable of causing invasive aspergillosis in patients with compromised immune systems. The fungus was long considered a purely asexual organism. However, a sexual cycle was reported in 2009, with methods described to induce mating under laboratory conditions. The presence of a sexual cycle now offers a valuable tool for classical genetic analysis of the fungus, such as allowing determination of whether traits of interest are mono‐ or poly‐genic in nature. For example, the sexual cycle is currently being exploited to determine the genetic basis of traits of medical importance such as resistance to azole antifungals and virulence, and to characterize the genes involved. The sexual cycle can also be used to assess the possibility of gene flow between isolates. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. This unit describes protocols for culturing of A. fumigatus and for inducing sexual reproduction between compatible MAT1‐1 and MAT1‐2 isolates of the species. The unit also provides working methods for harvesting sexual structures, isolating single‐spore progeny and confirming whether sexual recombination has occurred. © The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.