Immunochemical characterization of in vitro culture-derived antigens of Babesia bovis and Babesia bigemina.
Immunochemical characterization of in vitro culture-derived antigens of Babesia bovis and Babesia bigemina.
复制标题
牛巴贝虫和二联巴贝虫体外培养来源抗原的免疫化学特征。
DOI：
10.1016/s0304-4017(98)00095-8
复制
发表时间：
1998
影响因子：
2.6
通讯作者：
C. Brown
中科院分区：
文献类型：
--
作者：
L. Passos;L. Bell;C. Brown
在流行地区，牛巴贝斯虫和双线巴贝斯虫之间的交叉反应成为区分这两种感染的一个问题，因为这两个物种通常是相互关联的。为了确定可用作特异性诊断工具的候选蛋白质，采用免疫沉淀和丙烯酰胺凝胶电泳法分析了3个不同地理位置的牛黄杆菌种群(利斯莫尔、宽阳和墨西哥)和1个双链杆菌(墨西哥)的培养衍生成分。所采取的方法是基于对释放到培养上清液中的35S-蛋氨酸标记的寄生虫抗原的分析。对各种血清样本进行了测试，其中包括一组针对不同巴贝斯虫种群进行实验的小牛血清，一组在巴西野外自然感染的牛的血清样本，以及一组抗B。用牛黄单抗、间接荧光抗体试验、酶联免疫吸附试验和Western免疫印迹等方法鉴定。在牛分枝杆菌和双链杆菌培养上清液中分别检测到约28条和23条条带(分子量在200~14 kDa之间)，而在未感染的红细胞培养上清液(阴性对照)中未检测到条带。免疫沉淀分析表明牛群中存在抗原多样性，并鉴定出至少5个牛群共有的牛芽胞杆菌特异性抗原(分子量分别为80、72、58、38和24 kDa)和4个双链杆菌特异性抗原(分子量分别为240、112、50和29 kDa)。
Cross-reactivity between Babesia bovis and B. bigemina becomes a problem in discrimination of the two infections in endemic areas where the two species usually occur in association. With the aim of identifying candidate proteins for use as specific diagnostic tools, culture-derived components of three geographically different stocks of B. bovis (Lismore, Kwanyanga and Mexico) and one of B. bigemina (Mexico) were analyzed by immunoprecipitation using acrylamide gel electrophoresis. The approach taken was based on the analysis of35S-methionine-labelled parasite antigens released into culture supernatant. A variety of serum samples were tested, including a panel of calf sera experimentally produced against the different stocks of Babesia, serum samples from cattle naturally infected in the field in Brazil, and a panel of anti-B. bovis monoclonal antibodies, previously characterized by the indirect fluorescent antibody test, ELISA and Western immuno-blotting. Approximately 28 and 23 bands (with molecular weights ranging from 200 to 14 kDa) were detected in total protein profiles of B. bovis and B. bigemina culture supernatants, respectively, whereas no bands were seen in the uninfected red blood cell culture supernatant (negative control). The immunoprecipitation analysis showed antigenic diversity amongst the stocks of B. bovis and resulted in identification of at least five B. bovis specific antigens common to the three stocks (molecular weights of 80, 72, 58, 38 and 24 kDa) and four B. bigemina specific antigens (molecular weights of 240, 112, 50 and 29 kDa).
计算机物理通信。
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--