Differential gene expression in the small intestines of wildtype and W/WV mice
Differential gene expression in the small intestines of wildtype and W/WV mice
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DOI：
10.1046/j.1365-2982.2001.00256.x
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发表时间：
2001-04
影响因子：
3.5
通讯作者：
I. Takayama;Y. Daigo;S. Ward;K. Sanders;T. Yamanaka;M. Fujino
中科院分区：
文献类型：
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作者：
I. Takayama;Y. Daigo;S. Ward;K. Sanders;T. Yamanaka;M. Fujino
许多证明Cajal间质细胞（ICC）在胃肠道起搏和神经传递中的作用的证据来自对W/WV小鼠的研究。由于功能Kit蛋白的减少，这些动物在小肠中很少有起搏器ICC。我们检测了野生型和W/WV小鼠小肠中的基因表达。采用差异基因表达法研究了野生型和W/WV突变体空肠中RNA的表达。7个已知基因在野生型和W/WV小鼠中存在差异表达。在喂食和禁食的W/WV小鼠中，COX7B（细胞色素c氧化酶亚基VIIb）和SORCIN（编码多药耐药复合物，第4类）均被抑制。另外五个基因在W/WV小鼠中的表达增加：ADA（腺苷脱氨酶），MDH1（苹果酸脱氢酶），RPL‐8（核糖体蛋白L8）， SPTB2（谱蛋白，非红系，β亚基）和p6-5（编码磷酸化胆碱[PC] T细胞抑制因子[TsF]）。在禁食和喂食的动物中，差异表达是相同的，这表明差异与饮食状态无关。我们得出结论，几种基因在W/WV小鼠的小肠中差异表达，其中主要病变是起搏器ICC的丧失。差异基因显示可能有助于开发运动障碍的分子剖面，其中ICC丢失。
Much of the evidence demonstrating the role of interstitial cells of Cajal (ICC) in pacemaking and neurotransmission in the gastrointestinal tract comes from studies of W/WV mice. These animals have few pacemaker ICC in the small bowel due to reduced functional Kit protein. We examined gene expression in the small intestines of wildtype and W/WV mice. RNA expression in the jejunums of wildtype and W/WV mutants was studied using a differential gene expression method. Seven known genes were differentially expressed in wildtype and W/WV mice. COX7B (cytochrome c oxidase, subunit VIIb) and SORCIN (encoding multidrug‐resistance complex, class 4) were suppressed in both fed and fasted W/WV mice. Expression of another five genes was increased in W/WV mice: ADA (adenosine deaminase), MDH1 (malate dehydrogenase), RPL‐8 (ribosomal protein L8), SPTB2 (spectrin, nonerythroid, beta subunit), and p6–5 (encoding phosphorylcholine [PC] T‐cell suppressor factor [TsF]). Differential expression was the same in fasted and fed animals, suggesting that the differences were independent of the dietetic state. We conclude that several genes are differentially expressed in the small intestines of W/WV mice where the major lesion is loss of pacemaker ICC. Differential gene display may help develop a molecular profile of motility disorders in which ICC are lost.