Comparison of gene expression and activation of transcription factors in organoid-derived monolayer intestinal epithelial cells and organoids
Comparison of gene expression and activation of transcription factors in organoid-derived monolayer intestinal epithelial cells and organoids
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类器官来源的单层肠上皮细胞和类器官中基因表达和转录因子激活的比较
DOI：
10.1093/bbb/zbab136
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Sato Ryuichiro
中科院分区：
文献类型：
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作者：
Takahashi Yu;Inoue Yu;Kuze Keitaro;Sato Shintaro;Shimizu Makoto;Kiyono Hiroshi;Yamauchi Yoshio;Sato Ryuichiro
在体外，肠道器官类比常规使用的已建立的细胞系更好地代表活体肠道特性。然而，它们被维持在三维培养条件下，可能伴随着处理的复杂性。我们比较了人类器官衍生的二维培养的肠上皮细胞(IECS)和它们的亲代器官的性质。我们发现一些肠道标志物和功能基因的表达在单层肠内皮细胞和类器官之间没有明显的区别。我们进一步证实，它们的特定配体以剂量依赖的方式同样激活了肠道配体激活的转录调节因子。结果表明，培养条件并不显著影响来源于有机物质的单层IECs的基本性质，至少从基因表达调控的角度来看是这样。这将使它们能够作为新的生物工具用于研究人类肠道的生理功能。
Intestinal organoids better representin vivointestinal properties than conventionally used established cell linesin vitro. However, they are maintained in three-dimensional culture conditions that may be accompanied by handling complexities. We characterized the properties of human organoid-derived two-dimensionally cultured intestinal epithelial cells (IECs) compared with those of their parental organoids. We found that the expression of several intestinal markers and functional genes were indistinguishable between monolayer IECs and organoids. We further confirmed that their specific ligands equally activate intestinal ligand-activated transcriptional regulators in a dose-dependent manner. The results suggest that culture conditions do not significantly influence the fundamental properties of monolayer IECs originating from organoids, at least from the perspective of gene expression regulation. This will enable their use as novel biological tools to investigate the physiological functions of the human intestine.