Effect of repeat human blood feeding on Wolbachia density and dengue virus infection in Aedes aegypti
Effect of repeat human blood feeding on Wolbachia density and dengue virus infection in Aedes aegypti
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DOI：
10.1186/s13071-015-0853-y
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发表时间：
2015-04-24
影响因子：
3.2
通讯作者：
McGraw, Elizabeth A.
中科院分区：
文献类型：
--
作者：
Amuzu, Hilaria E.;Simmons, Cameron P.;McGraw, Elizabeth A.
背景资料：将内共生细菌Wolbachia引入埃及伊蚊种群是减少疾病传播的新方法。沃尔巴克氏体的存在限制了蚊子传播登革病毒（DENV）的能力，这种影响的强度似乎与蚊子中的沃尔巴克氏体密度相关。也有一些证据表明，沃尔巴克氏体密度可能会增加消费血粉。在这里，我们已经研究了是否多次血液喂养导致密度增加或相关的变化，在沃尔巴克氏体介导的阻断DENV.Methods：沃尔巴克氏体感染的埃及伊蚊蚊子线用于研究。有三个处理组;非血液喂养对照组，第二组喂养一次，第三组喂养两次人血。所有组用DENV-2口服感染，然后在感染后10-11天解剖它们的中肠和唾液腺。RNA/DNA同时提取每个组织和随后用于DENV RNA拷贝和沃尔巴克氏体密度quantization.Results：我们发现重复载体感受态实验之间的变化，没有明确的证据表明沃尔巴克氏体数量增加，无论是唾液腺或身体的其余部分与喂养，因此没有看到相应的改善DENV阻断。埃及伊蚊是“啜”食者，经常返回以获得血餐，因此重要的是评估重复血液喂养是否改善了基于沃尔巴克氏体的DENV阻断的功效。我们的工作表明，在实验室的背景下，当沃尔巴克氏体密度高，重复喂养不会提高阻塞，因此这种能力应该是稳定的饲养周期在外地。
Background: The introduction of the endosymbiotic bacterium, Wolbachia into Aedes aegypti populations is a novel approach to reduce disease transmission. The presence of Wolbachia limits the ability of the mosquito to transmit dengue virus (DENV) and the strength of this effect appears to correlate with Wolbachia densities in the mosquito. There is also some evidence that Wolbachia densities may increase following the consumption of a bloodmeal. Here we have examined whether multiple blood feeds lead to increases in density or associated changes in Wolbachia-mediated blocking of DENV.Methods: The Wolbachia infected Aedes aegypti mosquito line was used for the study. There were three treatment groups; a non-blood fed control, a second group fed once and a third group fed twice on human blood. All groups were orally infected with DENV-2 and then their midguts and salivary glands were dissected 10-11 days post infection. RNA/DNA was simultaneously extracted from each tissue and subsequently used for DENV RNA copies and Wolbachia density quantification, respectively.Results: We found variation between replicate vector competence experiments and no clear evidence that Wolbachia numbers increased in either the salivary glands or remainder of the body with feeding and hence saw no corresponding improvements in DENV blocking.Conclusions: Aedes aegypti are "sip" feeders returning often to obtain bloodmeals and hence it is important to assess whether repeat blood feeding improved the efficacy of Wolbachia-based DENV blocking. Our work suggests in the laboratory context when Wolbachia densities are high that repeat feeding does not improve blocking and hence this ability should likely be stable with respect to feeding cycle in the field.