Cytotoxity of two new coumarin derivatives isolated from Launaea mucronata
Cytotoxity of two new coumarin derivatives isolated from Launaea mucronata
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DOI：
10.1080/14786419.2015.1017492
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发表时间：
2016-01-01
影响因子：
2.2
通讯作者：
Mahmoud, Khaled
中科院分区：
文献类型：
--
作者：
El-Sharkawy, Eman R.;Mahmoud, Khaled
体外研究了 Launaea mucronata 地上部分的甲醇 (MeOH) 氯仿提取物对 HCT116、HepG2 和 MCF-7 细胞系的细胞毒性，结果 IC50 分别 = 20.0、18.6 和 14.30 mu g/mL。对甲醇提取物的氯仿部分进行进一步分馏，从而分离出两种新的香豆素化合物（6-异丁基香豆素和6-异丁基-7-甲基-香豆素）。新化合物的结构通过高场一维和二维核磁共振以及ESI-MS光谱阐明。低极性馏分揭示了其他已知三萜化合物的分离，这些化合物根据其光谱数据并与文献比较进行了鉴定。新型香豆素化合物对 MCF-7、HCT116 和 HepG2 细胞系表现出高细胞毒性。
The chloroform fraction of methanol (MeOH) extract of the aerial parts of Launaea mucronata was in vitro investigated for cytotoxicity against HCT116, HepG2 and MCF-7 cell lines, and resulted with IC50 = 20.0, 18.6 and 14.30 mu g/mL, respectively. The chloroform fraction of the MeOH extract was subjected to further fractionation, which led to the isolation of two new coumarin compounds (6-isobutyl coumarin and 6-isobutyl-7-methyl-coumarin). The structures of the new compounds were elucidated by high field 1D and 2D NMR and ESI-MS spectroscopies. Low polar fractions revealed the isolation of other known triterpene compounds which were identified according to its spectral data and comparison with the literature. New coumarin compounds show high cytotoxicity against MCF-7, HCT116 and HepG2 cell lines.