CONDITIONS DETERMINING INITIATION OF DNA SYNTHESIS IN 3T3-CELLS
CONDITIONS DETERMINING INITIATION OF DNA SYNTHESIS IN 3T3-CELLS
复制标题
DOI：
10.1073/pnas.66.1.204
复制
发表时间：
1970-01-01
影响因子：
11.1
通讯作者：
STOKER, MGP
中科院分区：
文献类型：
--
作者：
DULBECCO, R;STOKER, MGP
设计实验以区分由于接触或血清因子耗尽引起的生长抑制。用3 H-胸腺嘧啶核苷标记法和放射自显影法观察细胞的DNA合成情况。通过这种方式，可以描述单个细胞迁移到伤口的完整历史。结果表明，细胞间的拓扑关系在控制DNA合成的起始中起着重要作用。目前尚不清楚启动是否是通过从接触中释放或通过细胞利用血清因子的能力增加（因为它们的形状和活性变化）来促进的。
Experiments were designed to discriminate between inhibition of growth due to contacts or exhaustion of serum factors. The cell layer was wounded and the migrating cells were followed by time-lapse cinematography; DNA synthesis in the same cells was recognized by means of3H-thymidine labeling and radioautography. In this way, the complete history of individual cells migrating to the wound could be described. The results show that topographical relationships between cells play an important role in controlling initiation of DNA synthesis. It is still unclear whether initiation is promoted by release from contacts or by the increased ability of the cells to utilize serum factors because of their changes in shapes and activities.