Irradiation Sterilized Gelatin-Water-Glycerol Ternary Gel as an Injectable Carrier for Bone Tissue Engineering
Irradiation Sterilized Gelatin-Water-Glycerol Ternary Gel as an Injectable Carrier for Bone Tissue Engineering
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辐照灭菌明胶-水-甘油三元凝胶作为骨组织工程的可注射载体
DOI：
10.1002/adhm.201600749
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发表时间：
2017
影响因子：
10
通讯作者：
Gu Ning
中科院分区：
文献类型：
--
作者：
Zhao Yantao;Han Liwei;Yan Jun;Li Zhonghai;Wang Fuli;Xia Yang;Hou Shuxun;Zhong Hongbin;Zhang Feimin;Gu Ning
可注射明胶凝胶为填充骨缺损提供了一个有吸引力的选择。挑战是制造具有最佳凝胶化特性的明胶凝胶，其可以被辐照灭菌。在这里，明胶-水-甘油（GWG）凝胶被报道用作广谱可注射载体。这种三元凝胶甘油含量高，水含量低，在剂量（25 kGy）的伽马辐照后保持稳定。作为可注射凝胶，其在明胶浓度≤ 2.0%时在室温下保持粘稠溶液。它的储能模量急剧增加，并最终超过46-50 °C附近的损耗模量，表明从液体状状态转变为弹性凝胶状状态。该三元凝胶在储能模量（12-1700 Pa）方面变化显著，同时表现出窄的pH范围（5.58-5.66），这取决于明胶浓度。因此，它可以装载各种材料。与体内生理盐水和体外培养基相比，它具有高度的细胞相容性。当负载脱钙骨基质时，复合材料显示出良好的可注射性，并且在照射后具有优异的成骨性能。这些特征可归因于高亲水性和快速降解性。这些结果证明，该三元凝胶作为辐照灭菌和通用注射给药系统是有前途的。
Injectable gelatin gels offer an attractive option for filling bone defects. The challenge is to fabricate gelatin gels with optimal gelation properties, which can be irradiation sterilized. Here, a gelatin–water–glycerol (GWG) gel is reported for use as a broad‐spectrum injectable carrier. This ternary gel is high in glycerol and low in water, and remains stable after gamma irradiation at doses (25 kGy). As an injectable gel, it remains a viscous solution at gelatin concentrations ≤2.0%, at room temperature. Its storage modulus increases dramatically and eventually exceeds the loss modulus around 46–50 °C, indicating a transition from a liquid‐like state to an elastic gel‐like state. This ternary gel ranges significantly in terms of storage modulus (12–1700 Pa) while demonstrating a narrow pH range (5.58–5.66), depending on the gelatin concentration. Therefore, it can be loaded with a variety of materials. It is highly cytocompatible compared with saline in vivo and culture media in vitro. When loaded with demineralized bone matrix, the composites show favorable injectability, and excellent osteogenesis performance, after irradiation. These features can be attributed to high hydrophilicity and fast degradability. These findings justify that this ternary gel is promising as an irradiation‐sterilized and universal injectable delivery system.