CORRECTIVE RECOMBINATION OF MOUSE IMMUNOGLOBULIN-KAPPA ALLELES IN ABELSON MURINE LEUKEMIA VIRUS-TRANSFORMED PRE-B CELLS
CORRECTIVE RECOMBINATION OF MOUSE IMMUNOGLOBULIN-KAPPA ALLELES IN ABELSON MURINE LEUKEMIA VIRUS-TRANSFORMED PRE-B CELLS
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DOI：
10.1128/mcb.10.2.569
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发表时间：
1990-02-01
影响因子：
5.3
通讯作者：
VANNESS, BG
中科院分区：
文献类型：
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作者：
FEDDERSEN, RM;VANNESS, BG
小鼠免疫球蛋白.KAPPA 的先前表征。从鼠浆细胞瘤克隆的基因重排产物表明，单个.kappa 上可以发生两个重组事件。等位基因（R.M.Feddersen 和 B.G.Van Ness，Proc.Natl.Acad.Sci.USA 82：4792-4797，1985；M.A.Shapiro 和 M.Weigert，J.Immunol.139：3834-3839，1987）。确定单个 .kappa 上是否存在多次重组。等位基因可以通过纠正重组促进生产性 V-J 基因的形成，我们检查了几个 Abelson 鼠白血病病毒转化的前 B 细胞克隆，这些克隆重新排列了 .kappa。细胞培养过程中的位点。已重排一个.kappa 的克隆细胞系。等位基因非生产性地生长，同时维持种系构型中的其他等位基因，并生长次级亚克隆，随后表达.KAPPA。蛋白质，被分离并检查进一步的.kappa。重新排列。在该连续克隆中观察到全谱重排模式，包括种系等位基因的生产性和非生产性重组以及非生产性等位基因的二次重组。结果表明，纠正性 V-J 重组伴随着非生产性 .kappa 的位移。基因，以显着的频率出现（17 个产生 κ 的亚克隆中的 6 个）。观察到作为互惠产物的主要（非功能性）V-J 连接的删除和维持。
Previous characterization of mouse immunoglobulin .KAPPA. gene rearrangement products cloned from murine plasmacytomas has indicated that two recombination events can take place on a single .kappa. allele (R. M. Feddersen and B. G. Van Ness, Proc. Natl. Acad. Sci. USA 82:4792-4797, 1985; M. A. Shapiro and M. Weigert, J. Immunol. 139:3834-3839, 1987). To determine whether multiple recombinations on a single .kappa. allele can contribute to the formation of productive V-J genes through corrective recombinations, we have examined several Abelson murine leukemia virus-transformed pre-B-cell clones which rearrange the .kappa. locus during cell culture. Clonal cell lines which had rearranged one .kappa. allele nonproductively while maintaining the other allele in the germ line configuration were grown, and secondary subclones, which subsequently expressed .KAPPA. protein, were isolated and examined for further .kappa. rearrangement. A full spectrum of rearrangement patterns was observed in this sequential cloning, including productive and nonproductive recombinations of the germ line allele and secondary recombinations of the nonproductive allele. The results show that corrective V-J recombinations, with displacement of the nonproductive .kappa. gene, occur with a significant frequency (6 of 17 .kappa.-producing subclones). Both deletion and maintenance of the primary (nonfunctional) V-J join, as a reciprocal product, were observed.