Detecting Microbial Dysbiosis Associated with Pediatric Crohn Disease Despite the High Variability of the Gut Microbiota.
Detecting Microbial Dysbiosis Associated with Pediatric Crohn Disease Despite the High Variability of the Gut Microbiota.
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DOI：
10.1016/j.celrep.2015.12.088
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发表时间：
2016-02-02
期刊：
影响因子：
8.8
通讯作者：
Korolev KS
中科院分区：
文献类型：
--
作者：
Wang F;Kaplan JL;Gold BD;Bhasin MK;Ward NL;Kellermayer R;Kirschner BS;Heyman MB;Dowd SE;Cox SB;Dogan H;Steven B;Ferry GD;Cohen SA;Baldassano RN;Moran CJ;Garnett EA;Drake L;Otu HH;Mirny LA;Libermann TA;Winter HS;Korolev KS
宿主与其微生物群之间的关系很难理解，因为微生物群落及其环境都高度可变。我们开发了一套技术，以应对这一挑战的基础上人口动态和信息理论。这些方法鉴定了与小儿克罗恩病相关的其他细菌分类群，并且可以用比以前的统计方法更少的样品检测微生物群落的显著变化。我们还大大提高了基于粪便样本微生物群诊断的准确性，并发现微生物的生态位预测了其在克罗恩病中的作用。通常驻留在健康患者的管腔中的细菌在疾病中减少，而通常驻留在健康患者的粘膜上的细菌在疾病中增加。我们的研究结果还表明，与克罗恩病的关联是进化保守的，并提供了一个基于互信息的方法来可视化生态失调。
The relationship between the host and its microbiota is challenging to understand because both microbial communities and their environment are highly variable. We developed a set of techniques to address this challenge based on population dynamics and information theory. These methods identified additional bacterial taxa associated with pediatric Crohn's disease and could detect significant changes in microbial communities with fewer samples than previous statistical approaches. We also substantially improved the accuracy of the diagnosis based on the microbiota from stool samples and found that the ecological niche of a microbe predicts its role in Crohn’s disease. Bacteria typically residing in the lumen of healthy patients decrease in disease while bacteria typically residing on the mucosa of healthy patients increase in disease. Our results also show that the associations with Crohn’s disease are evolutionarily conserved and provide a mutual-information-based method to visualize dysbiosis.