Structural diversity and evolution of the Rf-1 locus in the genus Oryza
Structural diversity and evolution of the Rf-1 locus in the genus Oryza
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DOI：
10.1038/sj.hdy.6801026
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发表时间：
2007-11-01
期刊：
影响因子：
3.8
通讯作者：
Akagi, H.
中科院分区：
文献类型：
--
作者：
Kato, H.;Tezuka, K.;Akagi, H.
水稻中的Rf-1基因座在农业上是重要的，因为它恢复具有BT型细胞质雄性不育（CMS）的植物的育性。Rf-1基因座含有负责恢复生育力的基因的几个重复拷贝。我们分析了水稻属Rf-1基因座的基因组结构，以阐明该基因座的结构多样性和进化。我们在AA基因组的稻属物种中鉴定了6个与Rf-1同源的基因（Rf-1A至Rf-1F）。水稻Rf-1基因座结构复杂，至少可分为6个分类类型。重复基因及其侧翼区的核苷酸序列高度保守，表明复杂的Rf-1基因座结构是由重复基因之间的同源重组产生的。事实上，复杂的Rf-1基因座结构是共同的稻属物种，独立进化表明，复制的祖先Rf-1基因发生在水稻进化的早期和同源重组导致的Rf-1基因座结构的多样化。此外，每个重复基因的氨基酸序列在物种之间是保守的。这表明Rf-1位点的重复基因可能具有不同的功能，并可能通过控制水稻线粒体基因的表达来发挥作用，就像CMS的恢复一样。
The Rf-1 locus in rice is agriculturally important as it restores fertility in plants with BT-type cytoplasmic male sterility (CMS). The Rf-1 locus contains several duplicated copies of the gene responsible for restoration of fertility. We analyzed the genomic structure of the Rf-1 locus in the genus Oryza to clarify the structural diversity and evolution of the locus. We identified six genes (Rf-1A to Rf-1F) with homology to Rf-1 at this locus in Oryza species with an AA genome. The Rf-1 locus structures in the rice accessions examined were very complex and fell into at least six classification types. The nucleotide sequences of the duplicated genes and their flanking regions were highly conserved suggesting that the complex Rf-1 locus structures were produced by homologous recombination between the duplicated genes. The fact that complex Rf-1 locus structures were common to Oryza species that have evolved independently indicates that a duplication of the ancestral Rf-1 gene occurred early in rice evolution and that homologous recombination resulted in the diversification of Rf-1 locus structures. Additionally, the amino acid sequences of each duplicated gene were conserved between species. This suggests that the duplicated genes in the Rf-1 locus may have divergent functions and may act by controlling mitochondrial gene expression in rice as occurs in the restoration of CMS.