A comparison of chronic multi-channel cortical implantation techniques: manual versus mechanical insertion
A comparison of chronic multi-channel cortical implantation techniques: manual versus mechanical insertion
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DOI：
10.1016/j.jneumeth.2004.08.009
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发表时间：
2005-03-30
影响因子：
3
通讯作者：
Sloan, AM
中科院分区：
文献类型：
--
作者：
Rennaker, RL;Street, S;Sloan, AM
高密度多通道皮层内电极阵列允许研究人员同时记录神经元群体，以了解神经编码和可塑性。这些设备具有数十到数百个电极，这些电极的间隔在几平方毫米内。在插入过程中，高密度探针可以在穿透软脑膜之前压缩皮质几毫米。皮质组织的压缩已被证明会导致创伤性脑损伤（TBI），这可能是导致电极产量低和记录寿命缩短的主要原因。比较了两种在初级听皮层IV层长期植入多丝电极阵列的插入方法。构建了一种机械插入装置，能够快速插入电极阵列，而不会对大脑造成可见的压迫。从插入的阵列与机械装置记录的宽带点击和纯音的神经反应进行了比较，从手动插入方法使用显微操作器的结果。两种技术在手术后直接产生相似数量的活动通道，平均信噪比约为4.5。超过60%的植入机械插入装置的动物在第6周具有驱动活动，而手动插入阵列的动物在3周后没有表现出功能反应。本报告提供了初步证据，证明机械插入器械可防止皮质压缩，延长电极记录寿命。(C)2004年由Elsevier B.V.出版
High-density multi-channel intra-cortical electrode arrays allow researchers to record simultaneously from populations of neurons for the purpose of understanding neural coding and plasticity. These devices have tens to hundreds of electrodes spaced within a few square millimeters. During insertion, the high-density probes can compress the cortex several millimeters prior to breaking through the pia. Compression of cortical tissue has been demonstrated to result in traumatic brain injury (TBI) which may be a major contributor to low electrode yield and decreased recording longevity. Two insertion techniques for chronically implanting multi-wire electrode arrays in layer IV of primary auditory cortex were compared. A mechanical insertion device, capable of rapidly inserting the electrode array without visible compression of the brain, was constructed. The neural responses to broadband clicks and pure tones recorded from the arrays inserted with the mechanical device were compared to the results from a manual insertion method using a micromanipulator. Both techniques result in a similar number of active channels directly following surgery with a mean signal-to-noise ratio of approximately 4.5. Over 60% of the animals implanted with the mechanical insertion device had driven activity at week 6 whereas none of the animals with manually inserted arrays exhibited functional responses after 3 weeks. This report provides initial evidence that mechanical insertion devices, which prevent cortical compression, increase electrode recording longevity. (C) 2004 Published by Elsevier B.V.