EFFECT OF ASCORBIC ACID ON RHEUMATOID SYNOVIAL FLUID
EFFECT OF ASCORBIC ACID ON RHEUMATOID SYNOVIAL FLUID
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DOI：
10.1136/ard.23.4.295
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发表时间：
1964-01-01
影响因子：
27.4
通讯作者：
SANDSON, J
中科院分区：
文献类型：
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作者：
ABRAMS, E;SANDSON, J
方法（1）一般方法。6例双侧或单侧膝关节积液患者的膝关节共抽吸12次。根据Ropes、班尼特、Cobb、Bennox和Bennar（1959）修订的美国风湿病协会的标准，4例患者为典型的类风湿性关节炎，2例为疑似类风湿性关节炎。在关节抽吸时抽取静脉血样本。所有样品立即置于40 ℃的冰水中. (2)产物的制备（a）羟乙磺酸盐。通过Sandson和Hamerman（1962）的方法从正常滑液中制备纯化的透明质酸盐蛋白。通过Dische（1947）的己糖醛酸法（经Bowness（1957）修改）测定透明质酸盐的浓度。(b)蛋白质组分。如Kunkel和Trautman（1959）所述，通过在巴比妥缓冲液（0- 075 M，pH 8-6）中的聚氯乙烯块上进行区带电泳，制备正常血清和类风湿性滑液的蛋白组分。电泳后，将块切成1英寸的片段，并通过置换过滤洗脱蛋白质。通过Lowry、Rosebrough、Farr和Randall（1951）改良的Folin法测定各样品的蛋白质含量。合并适当的洗脱液，然后在火棉胶袋中通过超滤浓缩。在pH 8-6的巴比妥缓冲液（0.075M）中，用纸电泳法验证各组分的均一性。
Methods (1) General.-The knee joints of six patients with bilateral or unilateral knee joint effusions were aspirated a total of twelve times. By the criteria of the American Rheumatism Association as revised by Ropes, Bennett, Cobb, Jacox, and Jessar (1959) four patients had classical and two hadprobable rheumatoid arthritis. A venous blood sample was drawn at the timeof joint aspiration. All samples were immediately placed in ice water at 40 C.(2) Preparation of Products (a) Hyaluronate.-Purified hyaluronate protein was prepared by the method of Sandson and Hamerman (1962) from normal synovial fluids. The concentration of hyaluronate was determined by the hexuronic acid method of Dische (1947) as modified by Bowness (1957).(b) Protein Fractions.-Protein fractions of normal serum and of rheumatoid synovial fluid were prepared by zone electrophoresis on a polyvinyl chloride block in barbital buffer (0-075M, pH 8-6) as described by Kunkel and Trautman (1959). After electrophoresis the block was cut in i-inch segments and the protein eluted by displacement filtration. The protein content of each eluate was determined by the Folin method modified by Lowry, Rosebrough, Farr, and Randall (1951). The appropriate eluates were pooled and then concentrated by ultrafiltration in a collodion bag. The homogeneity of each fraction was verified by paper electrophoresis at pH 8-6 in barbital buffer (0 075M).