USE OF LIVE AND INACTIVATED SALMONELLA-ENTERITIDIS PHAGE TYPE-4 VACCINES TO IMMUNIZE LAYING HENS AGAINST EXPERIMENTAL-INFECTION
USE OF LIVE AND INACTIVATED SALMONELLA-ENTERITIDIS PHAGE TYPE-4 VACCINES TO IMMUNIZE LAYING HENS AGAINST EXPERIMENTAL-INFECTION
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DOI：
10.20506/rst.13.3.804
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发表时间：
1994-09-01
期刊：
影响因子：
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通讯作者：
ALOGAILY, ZH
中科院分区：
文献类型：
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作者：
NASSAR, TJ;ALNAKHLI, HM;ALOGAILY, ZH
四组 Dekalb Delta 商品蛋鸡（每组 8 只母鸡）单独饲养在隔离单元的笼子中。 8周龄时，第1组和第2组接种了9R鸡沙门氏菌活疫苗（9R live）。 18周龄时，第1组再次接种9R肠炎沙门氏菌活疫苗，第2组和第3组则接种肠炎沙门氏菌菌苗。 22 周龄时，第 2 组和第 3 组再次接种肠炎沙门氏菌菌苗。 第4组不接种疫苗，作为对照组。在24、27和30周龄时，使用不同浓度的肠炎沙门氏菌噬菌体4型和不同的接种途径对四组进行攻击。 每周收集鸡蛋、泄殖腔拭子和血液进行细菌学和血清学检查。接种组的蛋壳中分离出的攻击微生物和鸡蛋含量显着低于对照组。 体液抗体与鸡蛋或泄殖腔拭子中攻击微生物的脱落之间没有相关性。9R肠炎沙门氏菌活疫苗和肠炎沙门氏菌菌苗的联合疫苗接种方案比单独施用任何一种疫苗都能为产蛋母鸡提供更好的保护。 疫苗接种不应单独进行，而应与与兽医卫生和良好管理相关的其他措施结合起来。
Four groups of Dekalb Delta commercial layer hen (8 hens per group) were reared individually in cages in an isolation unit. At the age of 8 weeks, groups 1 and 2 were vaccinated with 9R Salmonella gallinarum live (9R live) vaccine. At the age of 18 weeks, group 1 was re-vaccinated with 9R live S. enteritidis vaccine, while groups 2 and 3 were vaccinated with S. enteritidis bacterin. At the age of 22 weeks, groups 2 and 3 were re-vaccinated with S. enteritidis bacterin. Group 4 was not vaccinated and was kept as a control group.At 24, 27 and 30 weeks of age, the four groups were challenged using various concentrations of S. enteritidis phage type 4 and various routes of inoculation. Eggs, cloacal swabs and blood were collected weekly for bacteriological and serological examination.Isolation of the challenge organism from the eggshells and egg contents of the vaccinated groups was significantly lower than with the control group. There was no correlation between humoral antibodies and the shedding of the challenge organism in eggs or cloacal swabs.A combined vaccination programme of 9R live S. enteritidis vaccine and S. enteritidis bacterin provided better protection to laying hens than either vaccine administered alone. Vaccination should never be performed alone but in conjunction with other measures related to veterinary hygiene and good management.