Interleukin-4 inhibits indoleamine 2,3-dioxygenase expression in human monocytes.
Interleukin-4 inhibits indoleamine 2,3-dioxygenase expression in human monocytes.
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DOI：
10.1182/blood.v83.5.1408.1408
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发表时间：
1994-03
期刊：
影响因子：
20.3
通讯作者：
T. Musso;G. Gusella;A. Brooks;D. Longo;L. Varesio
中科院分区：
文献类型：
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作者：
T. Musso;G. Gusella;A. Brooks;D. Longo;L. Varesio
吲哚胺2，3-双加氧酶(IDO)是一种依赖于黄素的酶，催化色氨酸转化为犬尿氨酸，由干扰素-γ诱导外周血单核细胞产生。白介素4(IL-4)是一种调节单核/巨噬细胞功能特性的细胞因子，我们研究了IL-4对IDO的影响。我们发现IL-4能抑制干扰素-γ诱导的人单核细胞IDO基因表达和IDO活性。当IL-4浓度为2U/m L时，抑制作用明显。这些结果首次证明细胞因子可以为IDO的表达提供负信号，并且IL-4可以影响色氨酸的分解代谢。
Indoleamine 2,3-dioxygenase (IDO), a flavin-dependent enzyme that catalyzes the conversion of tryptophan to kynurenine, is induced in peripheral blood mononuclear cells by interferon-gamma (IFN gamma). Interleukin-4 (IL-4) is a cytokine that modulates the functional properties of monocytes/macrophages, and we investigated the effects of IL-4 on IDO. We showed that IL-4 inhibited the induction of IDO mRNA and IDO activity by IFN gamma in human monocytes. The inhibitory effect was evident with as little as 2 U/mL of IL-4. These results provide the first evidence that a cytokine can provide a negative signal for IDO expression and that IL-4 can influence the catabolism of tryptophan.