Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring
Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring
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DOI：
10.1073/pnas.0605755103
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发表时间：
2006-08-29
影响因子：
11.1
通讯作者：
Ogura, Atsuo
中科院分区：
文献类型：
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作者：
Ogonuki, Narumi;Mochida, Keiji;Ogura, Atsuo
雄性生殖细胞的冷冻保存是保存对生物医学研究有价值的动物种类和品系的一种策略。我们测试了小鼠的雄性生殖细胞是否可以在没有冷冻保护的情况下通过简单地冷冻附睾体、睾丸或全身来进行冷冻保存。分离处死小鼠的生殖器官，在-80℃下冷冻1周至1年，然后收集精子和精子细胞注入成熟卵母细胞。无论测试品系(ICR和C57BL/6)如何，正常的幼崽都会出生。将附睾体和睾丸冷冻后空运到另一个实验室，可以产生近交系C57BL/6小鼠的幼鼠。从冷冻(-20℃)15年的雄性小鼠(BALB/c裸和C3H/He品系)体内提取的睾丸精子也可以通过微授精产生正常后代。因此，冷冻男性生殖器官或全身是保存男性生殖细胞的最简单方法。如果在永久冻土中发现雄性个体，就有可能恢复灭绝的物种。
Cryopreservation of male germ cells is a strategy to conserve animal species and strains of animals valuable to biomedical research. We tested whether mouse male germ cells could be cryopreserved without cryoprotection by simply freezing epididymides, testes, or whole bodies. The reproductive organs were isolated from killed mice and frozen for 1 week to 1 year at -80 degrees C before spermatozoa and spermatids were collected and injected into mature oocytes. Normal pups were born irrespective of strains tested (ICR and C57BL/6). Epididymides and testes frozen and transported internationally to another laboratory by air could produce pups of inbred C57BL/6 mice. Testicular spermatozoa retrieved from the bodies of male mice (BALB/c nude and C3H/He strains) that had been kept frozen (-20 degrees C) for 15 years could also produce normal offspring by microinsemination. Thus, freezing of either male reproductive organs or whole bodies is the simplest way to preserve male germ cells. Restoration of extinct species could be possible if male individuals are found in permafrost.