Effects of Aging on Autophagy After Experimental Intracerebral Hemorrhage
Effects of Aging on Autophagy After Experimental Intracerebral Hemorrhage
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DOI：
10.1007/978-3-7091-0693-8_18
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
Hua, Ya
中科院分区：
文献类型：
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作者：
Gong, Ye;He, Yangdong;Hua, Ya
脑出血（intracerebral hemorrhage，ICH）可导致老龄大鼠严重的脑损伤。自噬发生在脑出血后的大脑中，本研究探讨了衰老对脑出血后自噬的影响。老年（18-22个月）和年轻（4-6个月）雄性Fischer大鼠接受脑内注射100 μ L自体全血。在第7天处死大鼠进行Western印迹分析，以测量微管相关蛋白轻链-3（LC 3）（自噬体的生物标志物）和组织蛋白酶D（溶酶体生物标志物）。在ICH后11周处死大鼠进行脑组织学检查。还检查了神经功能缺损中的神经功能缺损相关变化。Western blotting结果显示，老年大鼠同侧基底节LC 3-I/LC 3-II转化率高于青年大鼠（p
Intracerebral hemorrhage (ICH) causes severe brain injury in aged rats. Autophagy occurs in the brain after ICH, and the present study examined the effects of aging on autophagy after ICH. Aged (18-22-month) and young (4-6-month) male Fischer rats received an intracerebral injection of 100-mu L autologous whole blood. Rats were killed at day 7 for Western blot analysis to measure microtubule-associated protein light chain-3 (LC3), a biomarker of autophagosome, and cathepsin D, a lysosomal biomarker. Rats were killed at 11 weeks after ICH for brain histology. Age-related changes in neurological deficits were also examined. Western blotting showed that the LC3-I/LC3-II conversion ratio in the ipsilateral basal ganglia was higher in aged compared to young rats (p