Successful spawning of cultured Penaeus monodon Fabricius after eyestalk ablation
Successful spawning of cultured Penaeus monodon Fabricius after eyestalk ablation
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眼柄切除后养殖斑节对虾成功产卵
DOI：
10.1016/0044-8486(77)90111-9
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发表时间：
1977
期刊：
影响因子：
4.5
通讯作者：
A. Santiago
中科院分区：
文献类型：
--
作者：
A. Santiago
初步实验结果表明，斑节对虾（Penaeus monodon）或“suppo”的圈养种群成功产卵，完成了生命周期，并产生了可存活的F1代幼虫。性成熟的雌性动物使用眼柄切除技术诱导性腺发育。发现去除一个眼柄足以诱导性腺发育，而双侧切除间接导致给药雌性动物的高死亡率。通过比较，成功产卵没有发生在unablated动物后9 months.鱼苗孵化的野生成鱼和饲养到25天后的幼虫进一步扣押在咸水实验池塘和饲养4个月。然后将1500只动物转移到海洋围栏。再过10个月后，选择600只动物进行实验;健康雌性动物单侧消融，并与100只雄性动物一起再放置9个月。1976年1月27日，7只23月龄的雌虫切除一个眼柄后，产卵成功，卵数、孵化率和幼虫存活率均优于海洋雌虫。目前尚不清楚实验的成功完全是由于消融的效果还是由于选择了合适的环境。目前正在继续努力在更严格控制的条件下诱导性腺发育，以克服产卵鱼供应不足和不可靠的问题。
Preliminary experiments resulted in successful spawning of captive stock of the prawnPenaeus monodon, or “sugpo”, to complete the life cycle and produce a viable F1generation of larvae. Gonadal development was induced using eyestalk ablation techniques on sexually mature females. Removal of one eyestalk was found to be sufficient to induce gonadal development, whereas bilateral ablation indirectly resulted in high mortality of the treated females. By comparison, successful spawning had not occurred in unablated animals after 9 months.Fry hatched from wild adult stock and reared to 25-day postlarvae were further impounded in brackishwater experimental ponds and reared for 4 months. A transfer of 1 500 animals were then made to marine pens. After a further 10 months, 600 animals were selected for the experiment; healthy females were ablated unilaterally and placed with 100 males for a further 9 months. Of the remaining 400 specimens, 200 were used as controls and the remainder ablated bilaterally, Seven of the 23-month old females with one eyestalk removed had spawned successfully by January 27, 1976.Egg numbers, hatching rate and larval survival compared favorably with data obtained from females of oceanic origin. It is not known if the success of the experiment was entirely due to the effect of ablation or to the choice of a suitable environment. Work is continuing to induce gonadal development under more closely controlled conditions to overcome the inadequate and unreliable supply of spawners.