Comparison of two methods of collecting induced sputum in asthmatic subjects
Comparison of two methods of collecting induced sputum in asthmatic subjects
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DOI：
10.1183/09031936.96.09122448
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发表时间：
1996-12-01
影响因子：
24.3
通讯作者：
Fahy, JV
中科院分区：
文献类型：
--
作者：
Gershman, NH;Wong, HH;Fahy, JV
我们以前报道的诱导痰的方法是收集吸入3%盐水气雾剂20分钟内产生的全部痰，这种方法存在着潜在的缺点，即唾液对诱导痰样本的贡献是相当大的和可变的。在本研究中，我们研究了在诱导痰过程中，分开收集唾液和痰是否是一种更好的诱导痰收集方法。在11名缓解期哮喘患者中，我们比较了四种诱导痰样本的容量、细胞总数和分类计数以及嗜酸性粒细胞阳离子蛋白(ECP)水平。两种方法(A方法)和两种方法(B方法)，受试者将唾液吐到一个容器中，然后向另一个容器咳嗽。我们发现，方法B获得的痰液量低于方法A(6.16+/-0.61vs20.1+/-2.7mL；P=0.003)，鳞状细胞百分比(34+/-4比47+/-6；P=0.023)。此外，B方法采集的标本中ECP水平较高(2 61+/-42vs145+/-2 6ngmL(-1)；p=0.0 1)，除中性粒细胞百分比低于B方法(37+/-4vs50+/-5%；p=0.019)外，其他非鳞状细胞分类计数相似。两种方法测定嗜酸性粒细胞百分率和ECP水平的重复性相似。我们得出结论，分别收集唾液和痰产生的诱导痰样本减少了唾液量，因此是一种更好的诱导痰收集方法。
The method that we have previously reported for sputum induction involves collecting the entire expectorate produced over a 20 min inhalation of 3% saline aerosol, This method presents the potential disadvantage of a considerable and variable salivary contribution to the induced sputum sample, In this study, we examined whether separate collection of saliva and sputum represents a better method for collecting induced sputum during sputum induction.In 11 stable asthmatics, we compared the volume, total and differential cell counts, and eosinophil cationic protein (ECP) levels in four induced sputum samples, two performed using our previous method (Method A) and two using another method (Method B) in which subjects spit saliva into one container before coughing sputum into another.We found that the volume of sputum obtained with Method B was lower than that obtained with Method A (6.16+/-0.61 vs 20.1+/-2.7 mL; p=0.003), as was the percentage of squamous cells (34+/-4 vs 47+/-6; p=0.023). In addition, the ECP levels in samples collected by Method B were higher (261+/-42 vs 145+/-26 ng mL(-1); p=0.01), The differential counts of nonsquamous cells were similar except for the percentage of neutrophils, which was lower in Method B (37+/-4 vs 50+/-5%; p=0.019). The repeatability of measurements of eosinophil percentages and of ECP levels was similar for the two methods.We conclude that separate collection of saliva and sputum yields induced sputum samples with reduced amounts of saliva and is, therefore, a better method for collecting induced sputum.