Three antimycobacterial metabolites identified from a marine-derived Streptomyces sp MS100061
Three antimycobacterial metabolites identified from a marine-derived Streptomyces sp MS100061
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从海洋来源的链霉菌中鉴定出三种抗分枝杆菌代谢物。
DOI：
10.1007/s00253-012-4681-0
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发表时间：
2013-05-01
影响因子：
5
通讯作者：
Zhang, Lixin
中科院分区：
文献类型：
--
作者：
Chen, Caixia;Wang, Jian;Zhang, Lixin
在从海洋天然产物库中筛选生物活性化合物的过程中，筛选到一株放线菌MS 100061，该菌株具有较强的抗牛分枝杆菌（Mycobacteriumbovis）、卡介苗（BacillusCalmette-Gu，rin）活性。根据形态学和16 S rDNA系统发育分析，该菌株属于链霉菌属（Streptomyces）。生物测定引导的分离产生了一种新的螺四酮，lobophorin G（1），以及两种已知的化合物，lobophorin A（2）和B（3）。通过多种波谱方法和文献对照进行结构鉴定。对化合物1-3进行抗BCG、抗结核和抗菌筛选，并显示出有效的抗BCG活性，其最小抑制浓度（MIC）值分别为1.56、1.56和0.78 μ g/ml，以及中等的抗结核分枝杆菌H37 Rv活性，其MIC值分别为32、32和16 μ g/ml。化合物1-3对枯草芽孢杆菌的MIC值分别为3.125、12.5和1.56 μ g/ml，表明具有很大的抗菌药物潜力。此外，这是首次报道的抗卡介苗和抗结核活性的lobophorins。
In the course of a screening program for bioactive compounds from a marine natural product library, a newly isolated Actinomycetes strain, designated as MS100061, exhibited strong anti-Mycobacterium bovis Bacillus Calmette-Gu,rin (BCG) activity. The strain belongs to the genus Streptomyces according to its morphological and 16S rDNA phylogenetic analysis. Bioassay-guided isolation resulted in a new spirotetronate, lobophorin G (1), together with two known compounds, lobophorins A (2) and B (3). The structures were elucidated by extensive spectroscopic methods and comparison with literatures. Compounds 1-3 were subjected to anti-BCG, antituberculosis, and antibacterial screening and exhibited potent anti-BCG activity with minimum inhibitory concentration (MIC) values of 1.56, 1.56, and 0.78 mu g/ml, respectively, and moderate anti-Mycobacterium tuberculosis H37Rv activity with MIC values of 32, 32, and 16 mu g/ml, respectively. The MIC values of compounds 1-3 against Bacillus subtilis were 3.125, 12.5, and 1.56 mu g/ml, respectively, indicating great potential for antibacterial drugs. In addition, this is the first report of the anti-BCG and antituberculosis activities of lobophorins.