Homologous genes of epidermal patterning factor regulate stomatal development in rice
Homologous genes of epidermal patterning factor regulate stomatal development in rice
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表皮图案因子同源基因调控水稻气孔发育
DOI：
10.1016/j.jplph.2019.01.010
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发表时间：
2019-03-01
影响因子：
4.3
通讯作者：
Liang, Yun-Kuan
中科院分区：
文献类型：
--
作者：
Lu, Jinjin;He, Jingjing;Liang, Yun-Kuan
气孔是叶子表面的微小孔隙，水通过它们以蒸汽形式进入大气并吸收二氧化碳进行光合作用。表皮模式因子 (EPF) 家族的信号肽调节拟南芥中的气孔发育和密度。水稻基因组中存在几种推定的 EPF/EPFL 同源物。为了了解它们可能参与气孔形成，在本研究中，我们生成了一系列转基因品系，包括报告启动子融合、下调和过度表达，并证明了不同基因型之间气孔密度的巨大差异，因为OsEPF1或OsEPF2的表达升高大大降低了水稻中的气孔密度，而OsEPF1或OsEPF2的异位过度表达显着降低了epf2和epflepf2突变株系的高气孔频率。拟南芥。相反，敲除OsEPFL9转录使转基因植物在水稻中比野生型植物具有更少的气孔，而过度表达水稻OsEPFL9基因可能导致拟南芥中气孔产生过多。总之，EPF/EPFL 的同系物以普遍高度保守的方式调节气孔发育，但双子叶植物和单子叶植物之间存在功能差异。
Stomata are microscopic pores on the surface of leaves through which water as vapor passes to the atmosphere and CO2 uptake for the photosynthesis. The signaling peptides of the epidermal patterning factor (EPF) family regulate stomatal development and density in Arabidopsis. Several putative homologs of EPF/EPFL exist in rice genome. To understand their possible involvement in stomatal formation, in this study we generated a series of transgenic lines including reporter promoter fusions, down-regulation and overexpression and demonstrated drastic differences in stomatal densities between different genotypes, as elevated expression of OsEPF1 or OsEPF2 greatly reduced stomatal density in rice, whereas ectopic overexpression of either OsEPF1 or OsEPF2 significantly decreased the high stomatal frequency of both mutant lines of epf2 and epflepf2 Arabidopsis. Conversely, knocking down OsEPFL9 transcription conferred transgenic plants with fewer stomata than WT in rice, whereas overexpressing rice OsEPFL9 gene could cause excessive production of stomata in Arabidopsis. In conclusion, homologs of EPF/EPFL regulate stomatal development in a generally highly conserved way yet there exist function distinctions between dicot and monocot plants.