Neuropeptides Amplify and Focus the Monoaminergic Inhibition of Nociception in Caenorhabditis elegans
Neuropeptides Amplify and Focus the Monoaminergic Inhibition of Nociception in Caenorhabditis elegans
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DOI：
10.1523/jneurosci.1324-13.2013
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发表时间：
2013-08-28
影响因子：
5.3
通讯作者：
Komuniecki, Richard
中科院分区：
文献类型：
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作者：
Hapiak, Vera;Summers, Philip;Komuniecki, Richard
单胺和神经肽相互作用来调节大多数行为。为了更好地理解这些相互作用，我们定义了酪胺 (TA)、章鱼胺和神经肽在抑制秀丽隐杆线虫厌恶行为中的作用。 TA 消除了由两个伤害性 ASH 感觉神经元介导的厌恶行为的血清素敏化，并需要在抑制性单胺能和肽能神经元上表达肾上腺素样、G α(q) 偶联的 TA 受体 TYRA-3。例如，TA 抑制需要肽能 ASI 感觉神经元中的 G alpha(q) 和 G alpha(s) 信号传导，一系列 ASI 神经肽激活参与运动决策的其他神经元上的神经肽受体。酪胺能抑制所需的 ASI 神经肽与章鱼胺能抑制所需的 ASI 神经肽不同，这表明单个单胺刺激 ASI 神经肽不同子集的释放。总之，这些结果表明，单胺的复杂体液混合物通过更局部的突触神经肽释放来调节伤害感受，并强调了秀丽隐杆线虫中伤害感受的酪胺能/章胺能抑制与哺乳动物中伤害感受的去甲肾上腺素能抑制之间的相似性，这也涉及抑制性肽能信号传导。
Monoamines and neuropeptides interact to modulate most behaviors. To better understand these interactions, we have defined the roles of tyramine (TA), octopamine, and neuropeptides in the inhibition of aversive behavior in Caenorhabditis elegans. TA abolishes the serotonergic sensitization of aversive behavior mediated by the two nociceptive ASH sensory neurons and requires the expression of the adrenergic-like, G alpha(q)-coupled, TA receptor TYRA-3 on inhibitory monoaminergic and peptidergic neurons. For example, TA inhibition requires G alpha(q) and G alpha(s) signaling in the peptidergic ASI sensory neurons, with an array of ASI neuropeptides activating neuropeptide receptors on additional neurons involved in locomotory decision-making. The ASI neuropeptides required for tyraminergic inhibition are distinct from those required for octopaminergic inhibition, suggesting that individual monoamines stimulate the release of different subsets of ASI neuropeptides. Together, these results demonstrate that a complex humoral mix of monoamines is focused by more local, synaptic, neuropeptide release to modulate nociception and highlight the similarities between the tyraminergic/octopaminergic inhibition of nociception in C. elegans and the noradrenergic inhibition of nociception in mammals that also involves inhibitory peptidergic signaling.