Modulation of MOG 37-50-specific CD8+ T cell activation and expansion by CD43
Modulation of MOG 37-50-specific CD8+ T cell activation and expansion by CD43
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DOI：
10.1016/j.cellimm.2006.06.007
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发表时间：
2006-03-01
影响因子：
4.3
通讯作者：
Evavold, Brian D.
中科院分区：
文献类型：
--
作者：
Ford, Mandy L.;Evavold, Brian D.
最近的一些报道描述了CD 8（+）T细胞在EAE中的效应作用。我们先前已经证明了MOG免疫后CD 43（-/-）小鼠的疾病发病率和严重程度降低，并将这种疾病进展的减弱归因于CD 43缺陷对CD 4（+）T细胞的影响。在这里，我们将这些研究扩展到研究CD 43缺失对MOG特异性CD 8（+）T细胞的影响。通过细胞内细胞因子和MHC四聚体染色证实，相对于野生型对照，在CD 43（-/-）小鼠中观察到免疫后MOG特异性CD 8（+）T细胞的频率降低。此外，与野生型CD 8（+）MOG致敏细胞的受体相比，从CD 43（-/-）小鼠过继转移CD 8（+）MOG 35-55致敏LN细胞导致EAE诱导显著减弱。对细胞内信号传导中间体的分析揭示了MOG特异性CD 8（+）T细胞在对抗原应答时磷酸化ERK的能力不足。这些结果表征了CD 43在自身反应性MOG特异性CD 8（+）T细胞活化和扩增过程中的重要作用。(c)2006年爱思唯尔公司All rights reserved.
Several recent reports have described an effector role for CD8(+) T cells during EAE. We have previously demonstrated reduced disease incidence and severity in CD43(-/-) mice following MOG immunization, and attributed this attenuation in disease progression to the effects of CD43 deficiency on CD4(+) T cells. Here, we extend those studies to examine the effects of the loss of CD43 on MOG-specific CD8(+) T cells. A reduced frequency of MOG-specific CD8(+) T cells following immunization was observed in CD43(-/-) mice relative to wild-type controls, as demonstrated by intracellular cytokine and MHC tetramer staining. In addition, adoptive transfer of CD8(+) MOG 35-55-primed LN cells from CD43(-/-) mice resulted in significantly attenuated EAE induction as compared to recipients of wild-type CD8(+) MOG-primed cells. Analysis of intracellular signaling intermediates revealed a deficiency in the ability of MOG-specific CD8(+) T cells to phosphorylate ERK in response to antigen. These results characterize an important role for CD43 during the activation and expansion of autoreactive MOG-specific CD8(+) T cells. (c) 2006 Elsevier Inc. All rights reserved.