The YPR153W gene is essential for the pressure tolerance of tryptophan permease Tat2 in the yeast Saccharomyces cerevisiae
The YPR153W gene is essential for the pressure tolerance of tryptophan permease Tat2 in the yeast Saccharomyces cerevisiae
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DOI：
10.1080/08957959.2017.1413367
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发表时间：
2018-01
影响因子：
2
通讯作者：
Goyu Kurosaka;F. Abe
中科院分区：
文献类型：
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作者：
Goyu Kurosaka;F. Abe
摘要在酿酒酵母中，已知25 MPa的静水压力是非致死的，但是显著地削弱了渗透酶Tat 2对色氨酸的摄取，从而抑制了需要来自培养基的色氨酸的菌株的生长。在这里，我们发现，缺乏YPR 153 W基因，到目前为止，其在酵母中的作用的特点很差，在10-25 MPa的菌株，需要色氨酸的生长造成了严重的不利影响。YPR 153 W的缺失导致Tat 2的压力诱导降解速率增加，表明Tat 2在25 MPa下在YPR 153 W缺失突变体中不稳定。TAT 2基因的过表达使缺失突变体能够在25 MPa下生长。这些结果表明，Ypr 153 w对于Tat 2在10-25 MPa压力下的稳定性和适当的运输功能是必需的。缩略语：Trp+：从头合成色氨酸的色氨酸原养型菌株的表型; Trp−：需要来自培养基的色氨酸的色氨酸营养缺陷型菌株的表型; YPR 153 W：一种特征性较差的酵母基因; Ypr 153 w：由YPR 153 W基因编码的蛋白质; ypr 153 w Δ：YPR 153 W基因的缺失突变体; Tat 2：高亲和力色氨酸通透酶; MCT 10：人芳香族氨基酸转运蛋白
ABSTRACT In the yeast Saccharomyces cerevisiae, hydrostatic pressure at 25 MPa is known to be nonlethal but significantly impairs the uptake of tryptophan by the permease Tat2, thereby inhibiting the growth of strains that require tryptophan from the medium. Here, we found that the lack of the YPR153W gene, so far poorly characterized for its role in yeast, caused a serious adverse effect on the growth at 10–25 MPa in the strain that required tryptophan. Deletion for YPR153W resulted in an increased rate of pressure-induced degradation of Tat2, suggesting that Tat2 is destabilized in the YPR153W deletion mutant at 25 MPa. Overexpression of the TAT2 gene enabled the deletion mutant to grow at 25 MPa. These results suggest that Ypr153w is essential for the stability and proper transport function of Tat2 under pressure at 10–25 MPa. ABBREVIATIONS: Trp+: the phenotype of tryptophan prototrophic strains that synthesize tryptophan de novo; Trp−: the phenotype of tryptophan auxotrophic strains that require tryptophan from the medium; YPR153W: an yeast poorly characterized gene; Ypr153w: a protein encoded by the YPR153W gene; ypr153wΔ: a deletion mutant for the YPR153W gene; Tat2: the high-affinity tryptophan permease; MCT10: a human aromatic amino acid transporter