The effect of E. coli endotoxins upon the fluorescein permeability of the blood-aqueous humour barrier.
The effect of E. coli endotoxins upon the fluorescein permeability of the blood-aqueous humour barrier.
复制标题
大肠杆菌内毒素对血-房水屏障荧光素渗透性的影响。
DOI：
10.1159/000136980
复制
发表时间：
1967
期刊：
影响因子：
--
通讯作者：
M. Winter
中科院分区：
文献类型：
--
作者：
G. Simon;L. Bertók;E. Morava;M. Winter
方法在本实验中，我们的目的是采用一种估算毛细血管通透性的方法，该方法本身不改变引起组胺类物质释放的通透性，同时也适用于分析通透性增加效应的时间条件。因此，我们选择了Amster和Huber(1)的方法。按此方法静脉注射0.5%荧光素钠溶液(每公斤体重0.3ml)。它可以穿透血-房水屏障，并可以在水溶液中用光学方法演示。作为毛细血管渗透性的一个定量特征，我们记录了染料在水溶液中‘饱和’的持续时间(水溶液中的颜色浓度达到接近最大值)。因此，“饱和时间”越短，渗透率就越高。实验选用本系大鼠120只，雌雄不拘，体重180~250g，肌注Nembutal(35 mg/kg体重，ip)。用Westphal等人(7)的方法对不同类型的大肠杆菌菌株进行内毒素提取。
MethodsIn our experiment we aimed to employ a method for the estimation of capillary permeability, which in itself does not alter the permeability inducing the liberation of histamine-like substances and at the same time it is suitable to analyse the time-conditions of the permeability increasing effect. Therefore we chose the method of Amster and Huber (1). According to this method the fluorescein-sodium in 0.5% solution (0.3 ml per kg body weight) was ad ministered intravenously. It penetrates through the blood-aqueous barrier and can be demonstrated with optical methods in the aqueous humour. As a quanti tative characteristic of capillary permeability we noted the duration of time during which the dye ‘saturated’the aqueous humour (the colour concentration of the aqueous humour reached an approximately maximal value). Thus, the shorter ‘saturation time’indicates an increased permeability. The experiments were performed on 120 albino rats of both sexes of our own breed, with a body weight of 180 to 250 g. The animals were anaesthetized with Nembutal (35 mg/kg body weight, ip). The endotoxin was extracted by the method of Westphal et al.(7) on various types of Escherichia coli strains.