Ficolin A and ficolin B are expressed in distinct ontogenic patterns and cell types in the mouse
Ficolin A and ficolin B are expressed in distinct ontogenic patterns and cell types in the mouse
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DOI：
10.1016/j.molimm.2004.11.019
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发表时间：
2005-07-01
影响因子：
3.6
通讯作者：
Fujita, T
中科院分区：
文献类型：
--
作者：
Liu, Y;Endo, Y;Fujita, T
无花果蛋白酶是一组特征在于存在胶原样和纤维蛋白原样结构域的蛋白质。三种人纤维胶凝蛋白中的两种，L-纤维胶凝蛋白和H-纤维胶凝蛋白，是与甘露糖结合凝集素相关丝氨酸蛋白酶（MASP）形成复合物的血清凝集素，并且在凝集素补体途径中起重要作用。另一种人纤维胶凝蛋白M-纤维胶凝蛋白是在单核细胞中表达的非血清型纤维胶凝蛋白。关于纤维胶凝蛋白的生理作用知之甚少。在这项研究中，我们描绘了个体发育和细胞类型，表达纤维胶凝蛋白的小鼠。RT-PCR分析表明，纤维胶凝蛋白A的表达模式与Masps的表达模式非常相似，表明这些分子可能作为凝集素补体途径的组成部分协同发挥作用。原位杂交结果表明，成年人肝脏和脾脏中表达纤维胶凝蛋白A mRNA的细胞类型均为巨噬细胞。Ficolin B表现出一种独特的个体发育模式，从胚胎肝脏转换到出生后的骨髓和脾脏。表达纤维胶凝蛋白B mRNA的细胞通过磁性分选和随后的骨髓细胞中的RT-PCR鉴定为属于骨髓细胞谱系。因此，纤维胶凝蛋白A和B的不同时空表达模式表明，这些分子在产前和产后阶段发挥不同的作用。(c)2004爱思唯尔有限公司保留所有权利。
Ficolins are a group of proteins characterized by the presence of collagen-like and fibrinogen-like domains. Two of three human ficolins, L-ficolin and H-ficolin, are serum lectins that form complexes with mannose-binding lectin-associated serine proteases (MASPs) and play important roles in the lectin complement pathway. The other human ficolin, M-ficolin, is a non-serum-type ficolin that is expressed in monocytes. Little is known about the physiological roles of ficolins. In this study, we delineated the ontogeny and cell types that express ficolins in mice. RT-PCR analysis showed that the expression pattern of ficolin A expression was closely similar to that of Masps, suggesting that these molecules may function in coordination as components of the lectin complement pathway. The cell types that express ficolin A mRNA in both adult liver and spleen were identified as macrophages by in situ hybridization. Ficolin B exhibited a distinct ontogeny pattern that switched from embryonic liver to postnatal bone marrow and spleen. The cells that express ficolin B mRNA were identified as belonging to the myeloid cell lineage by magnetic sorting and by subsequent RT-PCR in bone marrow cells. Thus, the different spatial-temporal expression patterns of ficolins A and B suggest that these molecules play distinct roles in the prenatal and postnatal stages. (c) 2004 Elsevier Ltd. All rights reserved.