A misaligned double-stranded RNA, poly(I).poly(C12,U), induces accumulation of 2',5'-oligoadenylates in mouse tissues.
A misaligned double-stranded RNA, poly(I).poly(C12,U), induces accumulation of 2',5'-oligoadenylates in mouse tissues.
复制标题
错位的双链 RNA，poly(I).poly(C12,U)，会诱导小鼠组织中 2,5-寡腺苷酸的积累。
DOI：
10.1016/0006-291x(86)90243-3
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Johnston,MI
中科院分区：
文献类型：
--
作者：
Hearl,WG;Johnston,MI
用2-200 μg的错配dsRNA poly（I）·poly（C12，U）静脉注射NIH Swiss小鼠，在脾、肝、肾和脑中2′，5 ′-寡腺苷酸合成酶被诱导3-2000倍。从这些组织中提取的2′，5 ′-寡腺苷酸的水平也升高，尽管提取的2′，5 ′-寡腺苷酸的量与存在的酶的量没有直接相关性。这些结果表明，错配聚合物的双链部分在细胞内存活并激活2′，5 ′-寡腺苷酸合成酶，并且dsRNA的水平可能有助于控制2′，5 ′-寡腺苷酸代谢。
2′,5′-Oligoadenylate synthetase was induced 3-2000-fold in spleen, liver, kidney and brain of NIH Swiss mice injected intravenously with 2–200 μg of the misaligned dsRNA, poly(I)·poly(C12,U). Levels of 2′,5′-oligoadenylates extracted from these tissues were also elevated, although the amount of 2′,5′-oligoadenylates extracted did not correlate directly with the amount of enzyme present. These results suggest that double-stranded portions of the misaligned polymer survived intracellularly and activated the 2′,5′-oligoadenylate synthetase, and that the level of dsRNA may contribute to the control of 2′,5′-oligoadenylate metabolism.