Sixteen years of evolution of human respiratory syncytial virus subgroup A in Buenos Aires, Argentina: GA2 the prevalent genotype through the years
Sixteen years of evolution of human respiratory syncytial virus subgroup A in Buenos Aires, Argentina: GA2 the prevalent genotype through the years
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DOI：
10.1016/j.meegid.2016.04.034
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发表时间：
2016-09-01
影响因子：
3.2
通讯作者：
Mistchenko, A. S.
中科院分区：
文献类型：
--
作者：
Viegas, M.;Goya, S.;Mistchenko, A. S.
人呼吸道合胞病毒（humanrespiratorysyncytialvirus，HRSV）是引起全球儿童急性下呼吸道感染（acutelowerrespiratorytrusinfections，LRTI）的主要病毒。近年来，已经报道了几项候选疫苗的临床前试验。正是在这个意义上，分子流行病学研究变得重要。了解疫苗接种前后的病毒传播模式可以深入了解控制策略的有效性。在这项工作中，我们分析了布宜诺斯艾利斯16年（1999 - 2014）期间HRSV-A的分子流行病学。通过生物信息学工具，我们分析了169个来自LRTI住院患儿的G糖蛋白基因序列。GA 2基因型占73.35%。GA5基因型在本地区共循环，直到2009年才不再检测到，2011年除外。最近全球出现的具有72-nt重复的ON1谱系增加了其频率，成为2014年在布宜诺斯艾利斯检测到的唯一谱系。通过对全球ON1菌株的离散地理分析，我们可以确定巴拿马可能是2010年6月20日MRCA的所在地;该谱系可能于2011年4月从西班牙引入阿根廷。该分析还显示，观察到ON1菌株的暂时和地理聚集，作为系统发育分支，与来自单个国家的菌株完全相关，然而，在我们来自三次爆发（2012 - 2014年）的44株ON1菌株中，我们还可以检测到来自美国，古巴，韩国和西班牙的后重新引入和循环。阿根廷ON1菌株的一个亚系的连续地理分析使我们能够建立，甚至在社区中也可能有一些菌株的局部聚集。这项工作显示了这种类型的生物信息学工具在未来疫苗监测网络中的潜力，以追踪新的遗传谱系在人群中的传播。(C)2016爱思唯尔B.V.保留所有权利。
Human respiratory syncytial virus (HRSV) is themain viral cause of acute lower respiratory tract infections (LRTI) in children worldwide. In recent years, several preclinical trials with vaccine candidates have been reported. It is in this sense that molecular epidemiological studies become important. Understanding viral dispersion patterns before and after the implementation of a vaccine can provide insight into the effectiveness of the control strategies. In this work we analyzed the molecular epidemiology of HRSV-A over a period of sixteen years (1999-2014) in Buenos Aires. By bioinformatic tools we analyzed 169 sequences of the G glycoprotein gene from hospitalized pediatric patients with LRTI. We found that GA2 was the most prevalent genotype (73.35%). GA5 genotype co-circulated in our region until 2009 when it was no longer detected, except in 2011. The recently globally emerging ON1 lineage with a 72-nt duplication increased its frequency to become the only lineage detected in Buenos Aires in 2014. By discrete phylogeographic analysis of global ON1 strains we could determine that Panama could be the location of the MRCA dated June 20, 2010; and this lineage could be introduced in Argentina from Spain in April 2011. This analysis also showed temporary and geographical clustering of ON1 strains observed as phylogenetic clades with strains exclusively associated from a single country, nevertheless among our 44 ON1 strains from three outbreaks (2012-2014) we could also detect posterior reintroductions and circulation from United States, Cuba, South Korea, and Spain. The continuous phylogeographic analysis of one sublineage of Argentine ON1 strains allowed us to establish that there could be a local clustering of some strains even in neighborhoods. This work shows the potential of this type of bioinformatic tools in the context of a future vaccine surveillance network to trace the spread of new genetic lineages in human populations. (C) 2016 Elsevier B.V. All rights reserved.