Dilution assessment of cervicovaginal secretions collected by vaginal washing to evaluate mucosal shedding of free human immunodeficiency virus
Dilution assessment of cervicovaginal secretions collected by vaginal washing to evaluate mucosal shedding of free human immunodeficiency virus
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DOI：
10.1128/cdli.4.5.624-626.1997
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发表时间：
1997-09-01
期刊：
影响因子：
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通讯作者：
Belec, L
中科院分区：
文献类型：
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作者：
Mohamed, AS;Becquart, P;Belec, L
10 mM浓度的锂不会干扰逆转录（RT）或PCR。用洗涤缓冲液中的锂（10 mM）作为稀释标记物，通过阴道捣碎法对宫颈阴道液进行取样，可用于通过定量RT-PCR准确测定HIV感染妇女中粘膜本身的上皮细胞处的非细胞HIV RNA的生殖器脱落。
A 10 mM concentration of lithium does not interfere with reverse transcription (RT) or PCR. Sampling of cervicovaginal fluid by vaginal mashing, with lithium (10 mM) in the washing buffer as a marker of dilution, may be utilized to accurately determine in HIV-infected women, by quantitative RT-PCR, the genital shedding of acellular HIV RNA at the let el of the mucosa itself.