Cloning of a DNA repeat element from horse: DNA sequence and chromosomal localization

Cloning of a DNA repeat element from horse: DNA sequence and chromosomal localization
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DOI:
10.1139/g95-150
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发表时间:
1995-12-01
期刊:
影响因子:
3.1
通讯作者:
Scott, IAS
Scott, IAS
中科院分区:
生物学3区
文献类型:
--
作者:
Broad, TE;Forrest, JW;Scott, IAS

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A DNA repeat element, revealed initially by digestion of horse DNA with TaqI, was cloned and characterized by Southern and in situ hybridization studies and nucleotide sequencing. The clone, e4/1, consisted of 32 tandem reiterations of a unit repeat of 21-22 bp, and produced multilocus DNA fingerprinting profiles that were useful for parentage analysis in horses. The tandem repeat element was shown by in situ hybridization to be localized in the centromeres of the acrocentric but not metacentric classes of horse chromosomes.