Diverse enzymatic activities mediate antiviral immunity in prokaryotes.
Diverse enzymatic activities mediate antiviral immunity in prokaryotes.
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DOI：
10.1126/science.aba0372
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发表时间：
2020-08-28
期刊：
影响因子：
--
通讯作者：
Zhang F
中科院分区：
文献类型：
--
作者：
Gao L;Altae-Tran H;Böhning F;Makarova KS;Segel M;Schmid-Burgk JL;Koob J;Wolf YI;Koonin EV;Zhang F
细菌和古细菌经常受到病毒和其他移动遗传因素的攻击，并依靠专用的抗病毒防御系统，例如限制性核酸内切酶和CRISPR来生存。病毒的巨大多样性表明，与目前所知的防御系统类型更多。通过系统的防御基因预测和异源重构，我们在这里发现了29种广泛的抗病毒基因盒，共有32％的所有测序细菌和古细菌基因组中介导了针对特定噬菌体的保护。这些系统结合了以前与抗病毒防御有关的酶活性，包括RNA编辑和retron卫星DNA合成。此外，我们在计算上预测了尚待表征的其他一组其他推定的防御基因。这些结果突出了微生物对病毒使用的巨大分子功能。
Bacteria and archaea are frequently attacked by viruses and other mobile genetic elements and rely on dedicated antiviral defense systems, such as restriction endonucleases and CRISPR, to survive. The enormous diversity of viruses suggests that more types of defense systems exist than are currently known. By systematic defense gene prediction and heterologous reconstitution, here we discover 29 widespread antiviral gene cassettes, collectively present in 32% of all sequenced bacterial and archaeal genomes, that mediate protection against specific bacteriophages. These systems incorporate enzymatic activities not previously implicated in antiviral defense, including RNA editing and retron satellite DNA synthesis. In addition, we computationally predict a diverse set of other putative defense genes that remain to be characterized. These results highlight an immense array of molecular functions that microbes use against viruses.
影响因子：
5.5
作者：
Anantharaman V;Makarova KS;Burroughs AM;Koonin EV;Aravind L
通讯作者：
Aravind L
影响因子：
--
作者：
Anantharaman, Vivek;Iyer, Lakshminarayan M.;Aravind, L.
通讯作者：
Aravind, L.