Phenotypic, physiological, and molecular evaluation of rice chilling stress response at the vegetative stage.
Phenotypic, physiological, and molecular evaluation of rice chilling stress response at the vegetative stage.
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DOI：
10.1007/978-1-62703-194-3_16
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Kim, Ki Young
中科院分区：
文献类型：
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作者：
de Los Reyes, Benildo G;Yun, Song Joong;Kim, Ki Young
水稻是一种对冷敏感的植物，在幼苗发育的早期和开花期间特别容易受到伤害。亚种间存在显着差异，水稻品种对稻瘟病的敏感度普遍低于大多数水稻品种。在大多数种植水稻的温带和亚热带国家，作物损害通常发生在幼苗发育的早期阶段，因为在晚春至初夏直播后的头几周，偶尔会出现寒流。不可逆转的伤害往往会导致幼苗死亡，或者如果作物在逆境中幸存下来，植物的活力和对病虫害的抵抗力就会严重受损。最近的生理和分子研究表明，氧化胁迫是水稻早期冷害的主要原因，水稻品种对氧化胁迫的反应在很大程度上是由氧化信号和防御相关基因的表达决定的。在这一章中，我们总结了基本的表型、生理和分子程序，可用于品种间差异反应的常规评估以及功能基因组学研究。
Rice is a chilling-sensitive plant that is particularly prone to injury during the early stages of seedling development and during flowering. Significant variation exists between subspecies with japonica cultivars generally being less sensitive than most indica cultivars. In most temperate and subtropical countries where rice is grown, crop damage often occurs during the early stages of seedling development due to occasional cold snaps coinciding with the first few weeks after direct seeding in late spring to early summer. Irreversible injuries often result in seedling mortality or if the crop survives a stress episode, plant vigor and resistance to pests and diseases are severely compromised. Recent physiological and molecular studies have shown that oxidative stress is the primary cause of early chilling injuries in rice and the differential responses of indica and japonica cultivars are defined to a large extent by gene expression related to oxidative signaling and defenses. In this chapter, we summarize basic phenotypic, physiological, and molecular procedures that can be adopted for routine evaluation of differential responses between cultivars as well as for functional genomics studies.