Surface Modified Nano-Electrospray Needles Improve Sensitivity for Native Mass Spectrometry
Surface Modified Nano-Electrospray Needles Improve Sensitivity for Native Mass Spectrometry
复制标题
表面改性纳米电喷雾针提高了天然质谱分析的灵敏度
DOI：
10.1021/jasms.2c00087
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发表时间：
2022
影响因子：
3.2
通讯作者：
Marty, Michael T.
中科院分区：
文献类型：
--
作者：
Kostelic, Marius M.;Hsieh, Chih-Chieh;Sanders, Henry M.;Zak, Ciara K.;Ryan, Jack P.;Baker, Erin S.;Aspinwall, Craig A.;Marty, Michael T.
天然质谱学(MS)和电荷检测-质谱学(CD-MS)已成为表征多种蛋白质和大分子复合体的通用工具。两者通常使用拉出的硼硅酸盐针的纳米电喷雾电离(NESI)，但已知一些分析物非特异性地吸附在玻璃上，这可能会降低灵敏度并限制数据的质量。为了提高天然MS和CD-MS的灵敏度，我们用包括聚乙二醇在内的惰性表面改性剂对NESI针进行了表面修饰。我们发现，表面修饰提高了蛋白质的天然MS和腺相关病毒衣壳的CD-MS的信号强度。基于机理比较，我们假设这种改善更有可能是由于涂层ESI针的流速增加，而不是非特异性吸附减少。在任何情况下，这些表面修饰的针提供了一种简单且廉价的方法来提高挑战性分析物的灵敏度。
Native mass spectrometry (MS) and charge detection-mass spectrometry (CD-MS) have become versatile tools for characterizing a wide range of proteins and macromolecular complexes. Both commonly use nanoelectrospray ionization (nESI) from pulled borosilicate needles, but some analytes are known to nonspecifically adsorb to the glass, which may lower sensitivity and limit the quality of the data. To improve the sensitivity of native MS and CD-MS, we modified the surface of nESI needles with inert surface modifiers, including polyethylene-glycol. We found that the surface modification improved the signal intensity for native MS of proteins and for CD-MS of adeno-associated viral capsids. Based on mechanistic comparisons, we hypothesize that the improvement is more likely due to an increased flow rate with coated ESI needles rather than less nonspecific adsorption. In any case, these surface-modified needles provide a simple and inexpensive method for improving the sensitivity of challenging analytes.