A novel fucose recognition fold involved in innate immunity
A novel fucose recognition fold involved in innate immunity
复制标题
DOI：
10.1038/nsb817
复制
发表时间：
2002-08-01
期刊：
影响因子：
--
通讯作者：
Amzel, LM
中科院分区：
文献类型：
--
作者：
Bianchet, MA;Odom, EW;Amzel, LM
欧洲鳗鲡凝集素（AAA）是一种存在于欧洲鳗鲡血清中的岩藻凝集素，参与动物天然免疫系统对细菌脂多糖的识别。由于AAA特异性识别H和刘易斯（a）血型的岩藻糖基化末端，因此已广泛用作血型分型和组织化学试剂。AAA包含一个新发现的碳水化合物识别结构域，存在于从细菌到脊椎动物的生物体的蛋白质中。AAA与α-L-岩藻糖复合物的晶体结构表征了整个凝集素家族的新折叠，鉴定了提供寡糖特异性结构决定因素的残基。这些残基的修饰解释了血清中不同的同种型如何提供不同的病原体特异性识别。
Anguilla anguilla agglutinin (AAA), a fucolectin found in the serum of European eel, participates in the recognition of bacterial liposaccharides by the animal innate immunity system. Because AAA specifically recognizes fucosylated terminals of H and Lewis (a) blood groups, it has been used extensively as a reagent in blood typing and histochemistry. AAA contains a newly discovered carbohydrate recognition domain present in proteins of organisms ranging from bacteria to vertebrates. The crystal structure of the complex of AAA with alpha-L-fucose characterizes the novel fold of this entire lectin family, identifying the residues that provide the structural determinants of oligosaccharide specificity. Modification of these residues explains how the different isoforms in serum can provide a diverse pathogen-specific recognition.