A Capillary Electrophoresis Electrospray Ionization-Mass Spectrometry Method using a Borate Background Electrolyte for the Fingerprinting Analysis of Flavonoids in Ginkgo biloba Herbal Supplements.
A Capillary Electrophoresis Electrospray Ionization-Mass Spectrometry Method using a Borate Background Electrolyte for the Fingerprinting Analysis of Flavonoids in Ginkgo biloba Herbal Supplements.
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DOI：
10.1039/c6ay00463f
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发表时间：
2016-04-28
期刊：
影响因子：
--
通讯作者：
Lunte CE
中科院分区：
文献类型：
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作者：
Johnson RT;Lunte CE
使用实验室构建的鞘液毛细管电泳-质谱接口开发了一种定性方法，用于植物样品中 14 种结构相似的黄酮、黄酮醇、黄酮酮和几种代表性糖苷的指纹分析。类黄酮的迁移顺序取决于类黄酮 B 环上存在的羟基数量、共轭程度、糖苷官能团的数量以及类黄酮与背景电解质形成稳定硼酸盐复合物的能力。类黄酮的母离子扫描得到 [M-H]−，但含有类黄酮的儿茶酚除外，其被检测为硼酸盐加合物。这些加合物可用于诊断以确定未知多酚化合物上是否存在儿茶酚基团。类黄酮苷和硼酸盐加合物的产物离子扫描通常会产生去质子化的苷元片段作为基峰，这可用于确认类黄酮的基本结构。通过分析银杏叶草药补充剂乙醇提取物中的类黄酮，证明了该方法的实用性。
A laboratory-built sheath liquid capillary electrophoresis-mass spectrometry interface was used to develop a qualitative method for fingerprinting analysis of 14 structurally similar flavones, flavonols, flavonones, and several representative glycosides in plant samples. The migration order of the flavonoids was dependent on a the number of hydroxyl groups present on the flavonoid B-ring, extent of conjugation, number of glycosidic functionalities, and ability of the flavonoid to form stable borate complexes with the background electrolyte. Parent ion scans of the flavonoids yielded [M-H]−, except for catechol containing flavonoids, which were detected as borate adducts. These adducts can be used diagnostically to determine the presence or absence of catechol groups on unknown polyphenolic compounds. Product ion scans of the flavonoid glycosides and borate adducts typically yielded the deprotonated aglycone fragment as the base peak, which could be used to confirm the base structure of the flavonoid. This method’s utility was demonstrated by analyzing flavonoids present in ethanolic extracts of Ginkgo biloba herbal supplements.