Induced sex reversal using an aromatase inhibitor, Fadrozole, in Atlantic halibut (Hippoglossus hippoglossus L.)
Induced sex reversal using an aromatase inhibitor, Fadrozole, in Atlantic halibut (Hippoglossus hippoglossus L.)
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DOI：
10.1016/j.aquaculture.2011.11.001
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发表时间：
2012-01-12
期刊：
影响因子：
4.5
通讯作者：
Norberg, Birgitta
中科院分区：
文献类型：
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作者：
Babiak, Joanna;Babiak, Igor;Norberg, Birgitta
全雌大西洋比目鱼(Hippoglossus parglossus L.)对该行业是可取的，因为在该物种中存在性别生长二型性。建立单性全雌性队列的第一步是产生性别颠倒的雌性(新雄性)。这项研究的目的是测试芳香酶抑制剂Fadrozole诱导大西洋比目鱼雄性化的效率。试验分三个剂量：100、500和700 mg/kg饲料，连续使用42-60天，对26-30 mm TL的断奶比目鱼鱼苗进行处理。所有测试剂量都被证明是有效的，导致高达97%的受检者睾丸发育。以17α-甲基睾酮(MT)为阳性对照。MT处理的鱼都是雄鱼。在所有组中均未观察到性腺发育异常。对11-酮睾酮(11kt)血浆水平的分析表明，试验组中大多数人的11kt水平是男性的典型水平，而对照组的男性或女性血浆11kt水平是典型的11kt水平。(C)2011爱思唯尔B.V.保留所有权利。
Production of all-female Atlantic halibut (Hippoglossus hippoglossus L.) is desirable for the industry because of sexual growth dimorphism existing in this species. The first step towards establishing monosex all-female cohorts is to produce sex-reversed females (neo-males). The objective of this study was to test the efficiency of an aromatase inhibitor, Fadrozole, to induce masculinisation in Atlantic halibut. Three doses of Fadrozole were tested: 100, 500 and 700 mg/kg of feed, applied for 42-60 days to weaned halibut fry of 26-30 mm TL. All tested doses proved to be efficient, resulting in the development of testes in up to 97% of examined individuals. Seventeen-a-methyltestosterone (MT) was used as a positive control. MT-treated fish were all males. No abnormalities in the development of gonads were observed in any of the groups. Analysis of 11-ketotestosterone (11KT) blood plasma levels showed that most of individuals in the experimental groups had 11KT levels typical for males while controls had 11KT levels typical for males or females. (C) 2011 Elsevier B.V. All rights reserved.