First identification of Neospora caninum infection in aborted bovine foetuses in China.
First identification of Neospora caninum infection in aborted bovine foetuses in China.
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DOI：
10.1016/j.vetpar.2007.07.013
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发表时间：
2007-10
影响因子：
2.6
通讯作者：
Wei Zhang;C. Deng;Qun Liu;Jing Liu;Ming Wang;K. Tian;X. Yu;D. Hu
中科院分区：
文献类型：
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作者：
Wei Zhang;C. Deng;Qun Liu;Jing Liu;Ming Wang;K. Tian;X. Yu;D. Hu
我国首次在流产牛胎组织中鉴定出犬新孢子虫感染。从 16 只母鼠身上收集了流产胎儿，其中 12 只母鼠的血清抗体滴度较高，使用 ELISA 检测试剂盒测定了犬新孢子虫。使用新孢子虫特异性 PCR 检测，从四个流产胎儿大脑中提取的 DNA 样本中扩增出犬新孢子虫的 Nc-5 基因，证实了流产胎儿中存在犬新孢子虫感染。组织学和免疫组织化学显示1名胎儿脑部存在直径25μm的厚壁（3μm）组织囊肿。胎儿的心脏和肺也发现非化脓性脑脊髓炎、局灶性出血、淋巴细胞浸润和出血等肝脏病变。因此，我们首次证实中华人民共和国流产牛胎儿感染犬新孢子虫。
The first identification of Neospora caninum infection in the tissues of aborted bovine foetuses in China is reported. Aborted foetuses were collected from 16 dams, and 12 of the dams had high serum antibody titres to N. caninum determined using an ELISA test kit. The Nc-5 gene of N. caninum was amplified from DNA samples extracted from brains of four aborted foetuses using a Neospora-specific PCR assay, confirming N. caninum infection in the aborted foetuses. Histology and immunohistochemistry showed thick-walled (3μm) tissue cyst in 25μm diameter in the brain of one foetus. Non-suppurative encephalomyelitis, focal haemorrhage, hepatic lesions consisted of lymphocyte infiltration and haemorrhage were also found in the heart and lung of the foetus. Thus, we have confirmed for the first time the infection of N. caninum in aborted foetuses of cattle in the People's Republic of China.