CIRCADIAN CLOCK-REGULATED AND PHYTOCHROME-REGULATED TRANSCRIPTION IS CONFERRED BY A 78 BP CIS-ACTING DOMAIN OF THE ARABIDOPSIS CAB2 PROMOTER
CIRCADIAN CLOCK-REGULATED AND PHYTOCHROME-REGULATED TRANSCRIPTION IS CONFERRED BY A 78 BP CIS-ACTING DOMAIN OF THE ARABIDOPSIS CAB2 PROMOTER
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DOI：
10.1046/j.1365-313x.1994.6040457.x
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发表时间：
1994-10-01
期刊：
影响因子：
7.2
通讯作者：
KAY, SA
中科院分区：
文献类型：
--
作者：
ANDERSON, SL;TEAKLE, GR;KAY, SA
拟南芥 CAB2 启动子用于定义受生物钟调节的末端基因组靶标。使用体内 cab::荧光素酶生物发光标记能够以前所未有的灵敏度和时间分辨率对活体幼苗中的嵌合构建体的表达进行分析。 CAB2启动子的-322到+1的解剖揭示了几个有趣的特征：证明323 bp片段包含至少一个强的一般阳性元件。正元件包含与蛋白质活性特异性结合的 ACGT 核心序列，称为 CUF-1，有助于高水平表达，但不是光敏色素或昼夜节律调节所必需的。此外，定义了一个 78 bp 的结构域，该结构域赋予异源启动子昼夜节律和光敏色素调节作用。 78 bp 调控域内的保守 GATA 序列与称为 CGF-1 的蛋白质因子特异性结合。 CGF-1 的结合特异性似乎与三螺旋 DNA 结合蛋白的 GT 家族有关。这些 DNA-蛋白质相互作用的作用从时钟和光敏色素调节方面讨论，以及它们作为光形态发生突变体定义的途径目标的相关性。
The Arabidopsis CAB2 promoter was used to define the terminal genomic targets that are subject to regulation by the circadian clock. An in vivo cab::luciferase bioluminescent marker was used to enable the assaying of the expression of chimeric constructs with unprecedented sensitivity and time resolution in living seedlings. Dissection of -322 to +1 of the CAB2 promoter has revealed several interesting features: it was demonstrated that the 323 bp fragment contains at least one strong general positive element. The positive element contains an ACGT core sequence specifically bound by a protein activity, termed CUF-1, and contributes to high level expression but is not required for phytochrome- or circadian-regulation. Moreover, a 78 bp domain was defined that confers both circadian- and phytochrome-regulation upon heterologous promoters. Conserved GATA sequences within the 78 bp regulatory domain are specifically bound by a protein factor designated CGF-1. The binding specificity of CGF-1 appears to be related to the GT-family of trihelix DNA-binding proteins. The role of these DNA-protein interactions is discussed in terms of clock- and phytochrome regulation, and their relevance as targets for pathways defined by photomorphogenic mutants.