A disposable, roll-to-roll hot-embossed inertial microfluidic device for size-based sorting of microbeads and cells.
A disposable, roll-to-roll hot-embossed inertial microfluidic device for size-based sorting of microbeads and cells.
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DOI：
10.1039/c6lc00215c
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发表时间：
2016-05
期刊：
影响因子：
6.1
通讯作者：
Xiao Wang;C. Liedert;R. Liedert;I. Papautsky
中科院分区：
文献类型：
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作者：
Xiao Wang;C. Liedert;R. Liedert;I. Papautsky
惯性微流控是近年来合成和生物微粒子高通量聚焦和分选的一个非常活跃的研究领域。然而，现有的惯性微流控装置总是依赖于具有高纵横比几何形状（通道宽度w h）的微通道来实现基于尺寸的微粒和细胞分选。该设备的简单LAR几何结构使使用R2R热压印的高通量制造成功。通过优化流动条件和通道尺寸，我们展示了使用低成本的一次性R2R芯片连续分选直径为15 μm和10 μm的混合微珠，分选效率为bb0.97%。我们进一步展示了基于尺寸的牛白细胞分选，展示了在我们的R2R芯片中处理真实细胞样本的能力。我们设想这种R2R热压印惯性微流控芯片将作为一种强大而低成本的一次性工具，用于工业应用中基于尺寸的合成微粒分选或细胞生物学研究和临床诊断中的细胞样品。
Inertial microfluidics has been a highly active area of research in recent years for high-throughput focusing and sorting of synthetic and biological microparticles. However, existing inertial microfluidic devices always rely on microchannels with high-aspect-ratio geometries (channel width w h) to achieve size-based sorting of microparticles and cells. The simple LAR geometry of the device enables successful high-throughput fabrication using R2R hot embossing. With optimized flow conditions and channel dimensions, we demonstrate continuous sorting of a mixture of 15 μm and 10 μm diameter microbeads with >97% sorting efficiency using the low-cost and disposable R2R chip. We further demonstrate size-based sorting of bovine white blood cells, demonstrating the ability to process real cellular samples in our R2R chip. We envision that this R2R hot-embossed inertial microfluidic chip will serve as a powerful yet low-cost and disposable tool for size-based sorting of synthetic microparticles in industrial applications or cellular samples in cell biology research and clinical diagnostics.