Construction of DGLA producing cell factory by genetic modification of Mucor circinelloides
Construction of DGLA producing cell factory by genetic modification of Mucor circinelloides
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卷枝毛霉基因改造DGLA生产细胞工厂的构建
DOI：
10.1186/s12934-019-1110-4
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发表时间：
2019-04-03
影响因子：
6.4
通讯作者：
Song, Yuanda
中科院分区：
文献类型：
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作者：
Khan, Md Ahsanul Kabir;Yang, Junhuan;Song, Yuanda
背景：二高-γ-亚麻酸（DGLA，20：3，n-6）是γ-亚麻酸（GLA，18：3，n-6）在δ-6延伸酶（D 6 E）或γ-亚麻酸延伸酶（GLELO）催化下的延伸产物。由于DGLA的营养价值和药用价值，工程产油微生物的构建引起了人们极大的兴趣。卷枝毛霉（Mucor circinelloides）是一种产GLA的丝状真菌，可以作为生产DGLA的有用工具。因此，我们在该真菌中过量表达了D 6 E（GLELO）基因，构建了DGLA生产细胞工厂。在旋毛虫中，分析了D 6 E（GLELO）基因的同源过表达。当该基因在M.结论：D 6 E（GLELO）基因在旋毛虫CBS277.49中的高表达是首次报道。以构建DGLA生产细胞工厂。这项工作为进一步研究建立了一个新的范围，以提高该真菌中DGLA的产量，特别是在其高产脂菌株WJ 11中。
Background:Dihomo-gamma linolenic acid (DGLA, 20:3, n-6) is the elongated product of Gamma linolenic acid (GLA, 18:3, n-6) catalyzed by the enzyme delta-6 elongase (D6E) or gamma linolenic acid elongase (GLELO). Construction of engineered oleaginous microbes have been attracting significant interest to produce DGLA because of its nutritional value and medicinal applications. Mucor circinelloides is a GLA producing filamentous fungus which can be a useful tool to produce DGLA. We have, therefore, overexpressed the D6E (GLELO) gene in this fungus to construct DGLA producing cell factory.Result:To produce DGLA in M. circinelloides, homologous overexpression of D6E (GLELO) gene was analyzed. When the gene was overexpressed in M. circinelloides CBS277.49, up to 5.72% DGLA was produced in this strain.Conclusion:To our knowledge, this is the first report describing the overexpression of D6E (GLELO) gene in M. circinelloides to construct DGLA producing cell factory. A new scope for further research has been established by this work for improved production of DGLA in this fungus, specifically in its high lipid-producing strain, WJ11.