Preponderant Mitotic Activity of Nonleukemic Cells Plays an Important Role in Failures to Detect Abnormal Clone in Childhood Acute Lymphoblastic Leukemia
Preponderant Mitotic Activity of Nonleukemic Cells Plays an Important Role in Failures to Detect Abnormal Clone in Childhood Acute Lymphoblastic Leukemia
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非白血病细胞的主要有丝分裂活性在未能检测到儿童急性淋巴细胞白血病异常克隆中发挥着重要作用
DOI：
10.1097/00043426-200307000-00004
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
P. Gaynon
中科院分区：
文献类型：
--
作者：
Shi;K. Weinberg;W. J. Joo;J. Quinn;J. Franklin;S. Siegel;P. Gaynon
在诊断时，超过三分之二的急性淋巴细胞白血病(ALL)儿童的骨髓原始细胞中发现克隆性染色体异常。然而，实际上，未能发现这些异常是经常的，通常归因于糟糕的骨髓采样，不充分的中期，和/或非白血病细胞中的优势有丝分裂活动。作者应用荧光原位杂交(FISH)技术对30例核型“正常”儿童ALL进行了重新检测，以探讨非白血病细胞的优势有丝分裂活动在未能发现克隆异常中的作用。用TEL/AML1融合基因探针和8、10号染色体着丝粒探针进行FISH检测t(12；21)易位和/或超二倍体。通过这种特殊设计的FISH分析，所有被检查的病例中有一半的核型“正常”被发现具有t(12；21)重排和/或超二倍体的异常克隆。与预期相反，作者发现，在检查了超过20个中期细胞的情况下，克隆异常的发生率(52%)高于分析了不到20个中期细胞的情况(44%)。这些发现表明，非白血病细胞的优势有丝分裂活性在常规细胞遗传学研究未能发现异常克隆的原因中起着重要作用。因此，核型“正常”的儿童所有患者都应该接受FISH检查，以排除t(12；21)和/或超二倍体克隆的存在。
At diagnosis, clonal chromosomal abnormalities are found in the bone marrow blasts in more than two thirds of children with acute lymphoblastic leukemia (ALL). Practically, however, failure to detect these abnormalities is frequent and usually attributed to poor marrow sampling, inadequate metaphases, and/or a preponderant mitotic activity among nonleukemic cells. The authors applied fluorescence in situ hybridization (FISH) techniques to re-examine 30 cases of karyotypically “normal” childhood ALL to explore the role of preponderant mitotic activities of nonleukemic cells in failures to detect clonal abnormalities. The FISH test were performed using TEL/AML1 fusion gene probe and the centromere probes for chromosome 8 and 10 to detect the t(12;21) translocation and/or hyperdiploidy. Half of the karyotypically “normal” ALL cases examined have been found to have abnormal clones with t(12;21) rearrangement and/or hyperdiploidy by this specially designed FISH assay. Contrary to expectation, the authors found a higher incidence (52%) of clonal abnormalities in cases where over 20 metaphases had been examined than in cases (44%) where fewer than 20 metaphases had been analyzed. These findings suggest that a preponderant mitotic activity of nonleukemic cells plays an important role in failures to detect an abnormal clone by conventional cytogenetic studies. Therefore, karyotypically “normal” childhood ALL patients should undergo FISH studies to rule out the presence of t(12;21) and/or hyperdiploid clone.
DOI：
10.1073/pnas.92.11.4917
发表时间：
1995-05-23
影响因子：
11.1
作者：
GOLUB, TR;BARKER, GF;GILLILAND, DG
通讯作者：
GILLILAND, DG
影响因子：
20.3
作者：
M. Harris;J. Shuster;A. Carroll;A. Look;M. Borowitz;W. Crist;R. Nitschke;J. Pullen;C. Steuber;V. Land
通讯作者：
M. Harris;J. Shuster;A. Carroll;A. Look;M. Borowitz;W. Crist;R. Nitschke;J. Pullen;C. Steuber;V. Land
儿童急性淋巴细胞白血病的细胞遗传学特征。
影响因子：
--
作者：
Katz,JA;Taylor,LD;Carroll,A;Elder,FF;Mahoney,DH
通讯作者：
Mahoney,DH