A Detailed Survey of Seed Coat Flavonoids in Developing Seeds of Brassica napus L.
A Detailed Survey of Seed Coat Flavonoids in Developing Seeds of Brassica napus L.
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DOI：
10.1021/jf903619v
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发表时间：
2010-05-26
影响因子：
6.1
通讯作者：
Routaboul, Jean-Marc
中科院分区：
文献类型：
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作者：
Auger, Bathilde;Marnet, Nathalie;Routaboul, Jean-Marc
原花青素是一种影响甘蓝型油菜粕消化率的种皮类黄酮。低pa系的发展与高质量的膳食以及油和蛋白质含量的增加有关，但需要更好地了解种子类黄酮。芸苔属植物成熟种子中的黄酮类化合物多为不溶性物质，目前对其含量的定性和定量研究很少。在本研究中，建立了8个黑籽甘蓝型油菜种皮黄酮类化合物的谱图，在种子发育过程中，可溶性黄酮类化合物以可溶性黄酮类化合物为主，而非可溶性黄酮类化合物以可溶性黄酮类化合物为主。采用液相色谱-电喷雾电离质谱联用技术（LC-ESI-MSn）对13种黄酮类化合物(-)-表儿茶素、5种原花青素（PCs仅由表儿茶素低聚物组成）和7种黄酮醇（槲皮素-3- o -葡萄糖苷、槲皮素-二己外苷、异鼠李素-3- o -葡萄糖苷、异鼠李素-二己外苷、异鼠李素-三己外苷和山奈酚-三己外苷）进行了鉴定和定量。这些黄酮醇衍生物首次在甘蓝型油菜种皮中被鉴定，异鼠李素-己糖苷硫酸盐和异鼠李素- sinapyl -三己糖苷在芸苔属植物种皮中积累了大量的PCs，在种子成熟过程中(-)-表儿茶素的溶剂溶性聚合物可达种皮重量的10%。此外，在8个黑籽基因型群体中，PC和黄酮醇含量均存在差异。本研究结果为低pc型甘蓝型油菜的育种提供了新的思路。
Proanthocyanidins (PAs) are seed coat flavonoids that impair the digestibility of Brassica napus meal. Development of low-PA lines is associated with a high-quality meal and with increased contents in oil and proteins, but requires better knowledge of seed flavonoids. Flavonoids in Brassica mature seed are mostly insoluble so that very few qualitative and quantitative data are available yet. In the present study, the profiling of seed coat flavonoids was established in eight black-seeded B. napus genotypes, during seed development when soluble flavonoids were present and predominated over the insoluble forms. Thirteen different flavonoids including (-)-epicatechin, five procyanidins (PCs which are PAs composed of epicatechin oligomers only) and seven flavonols (quercetin-3-O-glucoside, quercetin-dihexoside, isorhamnetin-3-O-glucoside, isorhamnetin-hexoside-sulfate, isorhamnetin-dihexoside, isorhamnetin-sinapoyl-trihexoside and kaempferol-sinapoyl-trihexoside) were identified and quantified using liquid chromatography coupled to electrospray ionization-mass spectrometry (LC-ESI-MSn). These flavonol derivatives were characterized for the first time in the seed coat of B. napus, and isorhamnetin-hexoside-sulfate and isorhamnetin-sinapoyl-trihexoside were newly identified in Brassica spp. High amounts of PCs accumulated in the seed coat, with solvent-soluble polymers of (-)-epicatechin reaching up to 10% of the seed coat weight during seed maturation. In addition, variability for both PC and flavonol contents was observed within the panel of eight black-seeded genotypes. Our results provide new insights into breeding for low-PC B. napus genotypes.