Single-cell fucosylation breakdown: Switching fucose to europium.
Single-cell fucosylation breakdown: Switching fucose to europium.
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单细胞岩藻糖基化分解：将岩藻糖转变为铕
DOI：
10.1016/j.isci.2021.102397
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发表时间：
2021-05-21
期刊：
影响因子：
5.8
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Liu Z;Liang Y;Zhou Y;Ge F;Yan X;Yang L;Wang Q
岩藻糖基化及其岩藻糖苷链特异性基序被认为是理解它们在细胞行为中的不同作用所必需的，但由于超敏感性和选择性的要求，它们的定量信息尚未完全披露。在此，我们报道了一种在硬电离电感耦合等离子体质谱(ICPMS)上将岩藻糖(Fuc)转换为稳定的Eu(Eu)同位素质量信号的方法。在细胞表面以代谢方式组装的叠氮岩藻糖使我们能够用一种用于欧盟信号增殖的炔定制噬菌体MS2衣壳纳米颗粒来标记它们，从而产生了有史以来最低的检测下限4.2zmolFuc。因此，在单个癌变的HepG2和癌旁的HL7702细胞上原位保存的连锁特异性岩藻糖基化基序的定量分解可以在单细胞电感耦合等离子体质谱平台上实现，从而确定它们在癌变过程中的作用。该方法被进一步应用于正常肝细胞和高、中、低分化的肝癌细胞的鉴别。在单细胞电感耦合等离子体质谱平台上，利用Eu修饰的MS2纳米颗粒单细胞裂解岩藻糖基连接特异性基序，实现了在单细胞电感耦合等离子体质谱平台上将轻巧的岩藻糖转换为稳定的Eu质量信号。化学；分析化学；生化
Fucosylation and its fucosidic linkage-specific motifs are believed to be essential to understand their distinct roles in cellular behavior, but their quantitative information has not yet been fully disclosed due to the requirements of ultra-sensitivity and selectivity. Herein, we report an approach that converts fucose (Fuc) to stable europium (Eu) isotopic mass signal on hard ionization inductively coupled plasma mass spectrometry (ICP-MS). Metabolically assembled azido-fucose on the cell surface allows us to tag them with an alkyne-customized Eu-crafted bacteriophage MS2 capsid nanoparticle for Eu signal multiplication, resulting in an ever lowest detection limit of 4.2 zmol Fuc. Quantitative breakdown of the linkage-specific fucosylation motifs in situ preserved on single cancerous HepG2 and paracancerous HL7702 cells can thus be realized on a single-cell ICP-MS platform, specifying their roles during the cancering process. This approach was further applied to the discrimination of normal hepatocellular cells and highly, moderately, and poorly differentiated hepatoma cells collected from real hepatocellular carcinoma tissues. Switching facile fucose to stable Eu mass signal on a single-cell ICP-MS platform Ever lowest LOD of 4.2 zmol FucAz was achieved using a Eu-decorated MS2 nanoparticle Single-cell breakdown of fucosidic linkage-specific motifs Discrimination of highly, moderately, and poorly differentiated HCC from normal ones Chemistry; Analytical Chemistry; Biochemistry
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影响因子：
2.5
作者：
Ghazarian, Haike;Idoni, Brian;Oppenheimer, Steven B.
通讯作者：
Oppenheimer, Steven B.
影响因子：
4.3
作者：
Fukushima, Keiko;Satoh, Takefumi;Yamashita, Katsuko
通讯作者：
Yamashita, Katsuko
影响因子：
15
作者：
Chaubard, Jean-Luc;Krishnamurthy, Chithra;Yi, Wen;Smith, David F.;Hsieh-Wilson, Linda C.
通讯作者：
Hsieh-Wilson, Linda C.
DOI：
10.1126/science.1198704
发表时间：
2011-05-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bendall SC;Simonds EF;Qiu P;Amir el-AD;Krutzik PO;Finck R;Bruggner RV;Melamed R;Trejo A;Ornatsky OI;Balderas RS;Plevritis SK;Sachs K;Pe'er D;Tanner SD;Nolan GP
通讯作者：
Nolan GP
影响因子：
64.5
作者：
Altschuler SJ;Wu LF
通讯作者：
Wu LF