Cloning and characterization of a novel G-protein-coupled receptor with homology to galanin receptors
Cloning and characterization of a novel G-protein-coupled receptor with homology to galanin receptors
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DOI：
10.1016/j.neuropharm.2004.02.004
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发表时间：
2004-06-01
影响因子：
4.7
通讯作者：
Schaller, HC
中科院分区：
文献类型：
--
作者：
Ignatov, A;Hermans-Borgmeyer, I;Schaller, HC
我们报告的识别，克隆和定位的人类和小鼠的直系同源物的一个新的G-蛋白偶联受体与甘丙肽受体，我们称之为甘丙肽受体样（GalRL）的同源性。GalRL基因定位于人染色体5 q32和小鼠染色体18133。北方印迹分析显示GalRL在人类和小鼠的中枢神经系统以及7天和15天大的小鼠胚胎中表达。在睾丸、肝脏、肾脏和胃等一些外周器官和组织中发现了少量水平。原位杂交实验表明GalRL在中枢神经系统中的主要表达，在缰复合体中具有独特的定位。在周围神经系统中，感觉神经节的单个神经元被标记。在胚胎发育过程中的表达是更广泛的神经系统中，除了背丘脑，杂交信号被检测到在大脑的其他领域，包括纹状体，蓝斑，和几个后脑核。甘丙肽对GalRL的弱激活表明内源性配体与甘丙肽共享结构特征。(C)2004爱思唯尔有限公司保留所有权利。
We report the identification, cloning, and localization of human and mouse orthologues of a new G-protein-coupled receptor with homology to galanin receptors, which we termed galanin-receptor like (GalRL). The genes of GalRL were localized to chromosome 5q32 in human and to 18133 in mouse. Northern blot analysis revealed expression of GalRL in the central nervous system of human and mouse and in 7- and 15-day-old mouse embryos. Minor levels were found in some peripheral organs and tissues, such as testis, liver, kidney and stomach. In situ hybridization experiments demonstrated predominant expression of GalRL in the central nervous system, with a distinct localization in the habenular complex. In the peripheral nervous system single neurons of sensory ganglia were labeled. During embryonal development the expression was more widespread in the nervous system, where in addition to the dorsal thalamus, hybridization signals were detected in other areas of the brain including the striatum, the locus coeruleus, and several hindbrain nuclei. A weak activation of GalRL by galanin suggests that the endogenous ligand shares structural features with galanin. (C) 2004 Elsevier Ltd. All rights reserved.