A 15N-aided artificial atmosphere gas flow technique for online determination of soil N2 release using the zeolite Köstrolith SX6.
A 15N-aided artificial atmosphere gas flow technique for online determination of soil N2 release using the zeolite Köstrolith SX6.
复制标题
15N 辅助人工大气气流技术，使用 Köstrolith SX6 沸石在线测定土壤 N2 释放量。
DOI：
10.1002/rcm.2722
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
C. Florian Stange
中科院分区：
文献类型：
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作者：
O. Spott;R. Russow;B. Apelt;C. Florian Stange
N2是土壤氮素转化过程中释放的主要气态氮化合物之一。由于它也是地球大气层的主要成分（78.08%体积），土壤氮释放的测定仍然是全面评价土壤气态氮损失的主要方法学挑战之一。常用的方法是基于C2 H2抑制技术、人工气氛或15 N示踪剂技术，并且被设计为封闭系统（非稳态）或气流系统（稳态）。这项工作的目的是升级目前的气流技术，使用人工气氛的15 N辅助测定土壤N2释放的同时与N2 O。一个15 N辅助人工大气气流的方法已经开发出来，它允许同时在线测定N2以及N2 O通量从开放的土壤系统（稳态）。这两种气体的通量可以在长的孵育期内连续测定，并具有高采样频率。N2选择性分子筛Köstrolith SX 6首次成功地进行了双氮收集试验。本文主要研究N2通量的测定。为了验证的目的，在有氧条件下（21和6体积%）培养了一个Haplic黑土的土壤团聚体。O2）和厌氧条件。只有在厌氧培养期间才释放出大量的N2（0.4和640.2 pmol N2 h（-1）g（-1）干土）。然而，一些N2的形成也发生在有氧培养。还发现，在进行反硝化过程中，引入的[NO3]-将比原始土壤[NO3]-更强烈地传递给微生物。
N2 is one of the major gaseous nitrogen compounds released by soils due to N-transformation processes. Since it is also the major constituent of the earth's atmosphere (78.08% vol.), the determination of soil N2 release is still one of the main methodological challenges with respect to a complete evaluation of the gaseous N-loss of soils. Commonly used approaches are based either on a C2H2 inhibition technique, an artificial atmosphere or a 15N-tracer technique, and are designed either as closed systems (non-steady state) or gas flow systems (steady state). The intention of this work has been to upgrade the current gas flow technique using an artificial atmosphere for a 15N-aided determination of the soil N2 release simultaneously with N2O. A 15N-aided artificial atmosphere gas flow approach has been developed, which allows a simultaneous online determination of N2 as well as N2O fluxes from an open soil system (steady state). Fluxes of both gases can be determined continuously over long incubation periods and with high sampling frequency. The N2 selective molecular sieve Köstrolith SX6 was tested successfully for the first time for dinitrogen collection. The presented paper mainly focuses on N2 flux determination. For validation purposes soil aggregates of a Haplic Phaeozem were incubated under aerobic (21 and 6 vol.% O2) and anaerobic conditions. Significant amounts of N2 were released only during anaerobic incubation (0.4 and 640.2 pmol N2 h(-1) g(-1) dry soil). However, some N2 formation also occurred during aerobic incubation. It was also found that, during ongoing denitrification, introduced [NO3]- will be more strongly delivered to microorganisms than the original soil [NO3]-.