Diversity of Staphylococcal Cassette Chromosome mec Structures in Methicillin-Resistant Staphylococcus epidermidis and Staphylococcus haemolyticus Strains among Outpatients from Four Countries
Diversity of Staphylococcal Cassette Chromosome mec Structures in Methicillin-Resistant Staphylococcus epidermidis and Staphylococcus haemolyticus Strains among Outpatients from Four Countries
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DOI：
10.1128/aac.00724-08
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发表时间：
2009-02-01
影响因子：
4.9
通讯作者：
Ruimy, Raymond
中科院分区：
文献类型：
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作者：
Ruppe, Etienne;Barbier, Francois;Ruimy, Raymond
在葡萄球菌中，甲氧西林（甲氧西林）耐药性（MR）是通过获得mecA基因介导的，该基因携带在大小和组成可变的葡萄球菌盒式染色体mec（SCCmec）上。MR在金黄色葡萄球菌中已被广泛研究，但对MR凝固酶阴性葡萄球菌（MR-CoNS）知之甚少。在这里，我们描述的多样性SCCmec结构的MR-CoNS从门诊患者生活在国家与对比的环境：阿尔及利亚，马里，摩尔多瓦和柬埔寨。他们的MR-CoNS鼻携带率分别为29、17、11和31%。分析了96株MR-CoNS菌株，包括75株（78%）表皮葡萄球菌菌株、19株（20%）溶血葡萄球菌菌株、1株（1%）人葡萄球菌菌株和1株（1%）柯尼葡萄球菌菌株。观察到18种不同的SCCmec类型，其中28种被鉴定为IV型（29%），25种为V型（26%），1种为III型（1%）。15株（44%）的SCCmec无法分型。34%的MR-CoNS菌株含有多个ccr拷贝。IV型和V型SCCmec与S.表皮葡萄球菌和表皮葡萄球菌。溶血性链球菌。MR-CoNS构成了社区中广泛且高度多样化的MR储库。
In staphylococci, methicillin (meticillin) resistance (MR) is mediated by the acquisition of the mecA gene, which is carried on the size and composition variable staphylococcal cassette chromosome mec (SCCmec). MR has been extensively studied in Staphylococcus aureus, but little is known about MR coagulase-negative staphylococci (MR-CoNS). Here, we describe the diversity of SCCmec structures in MR-CoNS from outpatients living in countries with contrasting environments: Algeria, Mali, Moldova, and Cambodia. Their MR-CoNS nasal carriage rates were 29, 17, 11, and 31%, respectively. Ninety-six MR-CoNS strains, comprising 75 (78%) Staphylococcus epidermidis strains, 19 (20%) Staphylococcus haemolyticus strains, 1 (1%) Staphylococcus hominis strain, and 1 (1%) Staphylococcus cohnii strain, were analyzed. Eighteen different SCCmec types were observed, with 28 identified as type IV (29%), 25 as type V (26%), and 1 as type III (1%). Fifteen strains (44%) were untypeable for their SCCmec. Thirty-four percent of MR-CoNS strains contained multiple ccr copies. Type IV and V SCCmec were preferentially associated with S. epidermidis and S. haemolyticus, respectively. MR-CoNS constitute a widespread and highly diversified MR reservoir in the community.