An inexpensive programmable optogenetic platform for controlled neuronal activation regimens in C. elegans
An inexpensive programmable optogenetic platform for controlled neuronal activation regimens in C. elegans
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DOI：
10.1063/1.5120002
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发表时间：
2020-03-01
影响因子：
6
通讯作者：
San-Miguel, Adriana
中科院分区：
文献类型：
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作者：
Crawford, Zachary;San-Miguel, Adriana
在秀丽隐杆线虫中，光遗传学刺激已被广泛用于评估神经元功能、控制动物运动或测定电路对受控刺激的反应。大多数研究都是在单个动物身上进行的，需要高端组件，如激光和快门。我们提出了一个可访问的平台，使控制光遗传刺激的C。elegans在两种模式：单一动物刺激与运动跟踪和整个群体刺激神经元运动方案。该系统由可访问的电子组件组成：高功率发光二极管，Arduino板和继电器与MATLAB集成，以实现可编程的光遗传学刺激方案。该系统在自由移动的动物中提供光遗传学刺激的灵活性，同时提供光遗传学驱动的运动响应的定量信息。我们通过刺激C. elegans和收缩反应的定量评估。此外，我们测试了突触可塑性耦合的整个人口的刺激模式与突触强度的测量使用涕灭威测定，其中明确的变化后，观察到的神经元运动方案的突触强度。该平台由廉价的组件组成，同时提供高端系统的照明强度，这需要昂贵的激光器，快门或自动化平台。该平台不需要移动部件，但提供了刺激方案的灵活性。
In Caenorhabditis elegans, optogenetic stimulation has been widely used to assess neuronal function, control animal movement, or assay circuit responses to controlled stimuli. Most studies are performed on single animals and require high-end components such as lasers and shutters. We present an accessible platform that enables controlled optogenetic stimulation of C. elegans in two modes: single animal stimulation with locomotion tracking and entire population stimulation for neuronal exercise regimens. The system consists of accessible electronic components: a high-power light-emitting diode, Arduino board, and relay are integrated with MATLAB to enable programmable optogenetic stimulation regimens. This system provides flexibility in optogenetic stimulation in freely moving animals while providing quantitative information of optogenetic-driven locomotion responses. We show the applicability of this platform in single animals by stimulation of cholinergic motor neurons in C. elegans and quantitative assessment of contractile responses. In addition, we tested synaptic plasticity by coupling the entire-population stimulation mode with measurements of synaptic strength using an aldicarb assay, where clear changes in synaptic strength were observed after regimens of neuronal exercise. This platform is composed of inexpensive components, while providing the illumination strength of high-end systems, which require expensive lasers, shutters, or automated stages. This platform requires no moving parts but provides flexibility in stimulation regimens.