Mutagenicity and oxidative damage induced by an organic extract of the particulate emissions from a simulation of the deepwater horizon surface oil burns
Mutagenicity and oxidative damage induced by an organic extract of the particulate emissions from a simulation of the deepwater horizon surface oil burns
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DOI：
10.1002/em.22085
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发表时间：
2017-04-01
影响因子：
2.8
通讯作者：
Gullett, Brian K.
中科院分区：
文献类型：
--
作者：
DeMarini, David M.;Warren, Sarah H.;Gullett, Brian K.
与 2010 年 4 月 20 日墨西哥湾开始的深水地平线爆炸和石油排放有关的石油火灾排放物当时仅进行了有限程度的化学分析，但结果显示可在体外和小鼠体内诱导氧化损伤。为了扩展这项工作，我们燃烧了漂浮在海水上的石油，并对排放物进行了广泛的化学分析（Gullett 等人，Marine Pollut Bull，正在出版，）。在这里，我们研究了这些排放物中空气动力学尺寸为 2.5 μ m (PM2.5) 的颗粒材料的二氯甲烷提取物在体外诱导人肺细胞氧化损伤的能力以及 6 种沙门氏菌菌株的致突变性。提取物的可提取有机物质（EOM）百分比为7.0%，在20μg EOM/ml下暴露4小时后，BEAS-2B细胞中血红素加氧酶（HMOX1）基因的表达增加。然而，根据细胞外通量分析测量，提取物并没有改变线粒体呼吸速率。提取物在 TA100 +S9 中的致突变性最强，表明多环芳烃 (PAH) 的作用，反映了排放物中 PAH 的高浓度（1 g/kg 消耗的油）。 TA98 +S9 中提取物的致突变性排放因子为 1.8 +/- 0.1 x 10(5) 回复体/兆焦耳（热），大于柴油废气，与木材和塑料露天燃烧的数量级相当。因此，燃烧油中的 PM2.5 产生的有机物可诱导人体气道上皮细胞发生氧化反应，并且具有高度致突变性。环境。摩尔。诱变剂。 58:162-171，2017 年。(c) 2017 年 Wiley 期刊公司。
Emissions from oil fires associated with the Deepwater Horizon explosion and oil discharge that began on April 20, 2010 in the Gulf of Mexico were analyzed chemically to only a limited extent at the time but were shown to induce oxidative damage in vitro and in mice. To extend this work, we burned oil floating on sea water and performed extensive chemical analyses of the emissions (Gullett et al., Marine Pollut Bull, in press, ). Here, we examine the ability of a dichloromethane extract of the particulate material with an aerodynamic size2.5 mu m (PM2.5) from those emissions to induce oxidative damage in human lung cells in vitro and mutagenicity in 6 strains of Salmonella. The extract had a percentage of extractable organic material (EOM) of 7.0% and increased expression of the heme oxygenase (HMOX1) gene in BEAS-2B cells after exposure for 4 hr at 20 mu g of EOM/ml. However, the extract did not alter mitochondrial respiration rate as measured by extracellular flux analysis. The extract was most mutagenic in TA100 +S9, indicative of a role for polycyclic aromatic hydrocarbons (PAHs), reflective of the high concentrations of PAHs in the emissions (1 g/kg of oil consumed). The extract had a mutagenicity emission factor of 1.8 +/- 0.1 x 10(5) revertants/megajoule(thermal) in TA98 +S9, which was greater than that of diesel exhaust and within an order of magnitude of open burning of wood and plastic. Thus, organics from PM2.5 of burning oil can induce oxidative responses in human airway epithelial cells and are highly mutagenic. Environ. Mol. Mutagen. 58:162-171, 2017. (c) 2017 Wiley Periodicals, Inc.