High-resolution functional proteomics by active-site peptide profiling
High-resolution functional proteomics by active-site peptide profiling
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DOI：
10.1073/pnas.0501205102
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发表时间：
2005-04-05
影响因子：
11.1
通讯作者：
Patricelli, MP
中科院分区：
文献类型：
--
作者：
Okerberg, ES;Wu, JY;Patricelli, MP
从基因组测序项目预测的大量蛋白质的表征和功能注释构成了重大的科学挑战。尽管已经开发了几种蛋白质组学技术来定量蛋白质的丰度，但这些方法提供的关于蛋白质功能的信息很少。在这里，我们提出了一个无凝胶平台，允许超灵敏，定量和高分辨率分析蛋白质组中的蛋白质活性，包括高度有问题的样品，如未稀释的血浆。我们证明了这个平台对于发现疾病相关酶活性和靶向这些蛋白质的特异性抑制剂的价值。
Characterization and functional annotation of the large number of proteins predicted from genome sequencing projects poses a major scientific challenge. Whereas several proteomics techniques have been developed to quantify the abundance of proteins, these methods provide little information regarding protein function. Here, we present a gel-free platform that permits ultrasensitive, quantitative, and high-resolution analyses of protein activities in proteomes, including highly problematic samples such as undiluted plasma. We demonstrate the value of this platform for the discovery of both disease-related enzyme activities and specific inhibitors that target these proteins.